The kanamycin resistant pUC vectors pHSG298 and pHSG299 contain the kanamycin resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These kanamycin resistant pUC vectors also contain a pUC19-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter in both the pHSG298 and pHSG299 vectors.
Both pHSG298 and pHSG299 can be analyzed via dideoxy DNA sequencing using an M13 primer. Large recombinant chloramphenicol resistant pUC vectors may be analyzed using Takara's Deletion Kit for Kilo-Sequencing (Cat. #6030).