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Products >  Cloning_and_Competent_Cells >  Cloning_Vectors >  Kanamycin_Resistant_pUC_Vectors

Kanamycin Resistant pUC Vectors

The kanamycin resistant pUC vectors pHSG298 and pHSG299 contain the kanamycin resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These kanamycin resistant pUC vectors also contain a pUC19-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter in both the pHSG298 and pHSG299 vectors.

Both pHSG298 and pHSG299 can be analyzed via dideoxy DNA sequencing using an M13 primer. Large recombinant chloramphenicol resistant pUC vectors may be analyzed using Takara's Deletion Kit for Kilo-Sequencing (Cat. #6030).

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Features

  • Form: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA

Applications

  • lac promoter-enabled target gene cloning and expression
  • DNA sequencing using an M13 primer
  • Long DNA sequencing using Takara's Deletion Kit for Kilo-Sequencing

Purity

  • >70% double-stranded, covalently closed circular form (RF1) DNA
  • Multiple cloning site sequence-verified via dideoxy DNA sequencing

Storage

–20°C

Notes

Except for the MCS, pHSG298 has the same general sequence as pHSG299 with the following exception:

pHSG298 lacks a G at position 1,446 in the sequence of pHSG299 that is registered with GenBank.

The pHSG299 DNA (Cat.# 3299) sequence differs from the sequence of pHSG299 registered with GenBank at the following positions:

1663 C –> deletion

1808 C –> T

2228–2229 AT –> TA

2474 G –> A

2667–2668 GA –> deletion

Concentration

250–1,000 µg/mL

Chain Length

  • pHSG298: 2,675 bp
  • pHSG299: 2,673 bp

GenBank

 Entry NameAccession No.
pHSG299SYNHSG299M19415

 
 
Products
Cat. # Product Contents Size Price Units Select
3298 pHSG 298 DNA 25 ug $47.00
3299 pHSG 299 DNA 25 ug $47.00
 

 

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6030 Deletion Kit for Kilo-Sequencing 5 Rxns $169.00
 
 
 

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