Cloning Vectors—In-Fusion Ready Fluorescent Protein Vectors
To simplify the construction and testing of fluorescent protein fusions, we provide prelinearized versions of our popular DsRed-Monomer-N1/C1 and AcGFP1-N1/C1 cloning vectors for fast, efficient PCR cloning with In-Fusion. Simply use appropriate gene-specific PCR primers encoding 15 nucleotide 5' extensions homologous to the ends of these prelinearized vectors to amplify your gene of interest. A single insert can be cloned directly into any one of these four In-Fusion Ready vectors; saving time and allowing parallel cloning of fusion proteins with different colors at either the N- or C-terminus. This is important when the ability of a protein of interest to function either as an N- or C-terminal fusion is unknown.