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Products >  Cloning_and_Competent_Cells >  Cloning_Vectors >  Ecoli-Aspergillus_Shuttle_Vectors

Pyrithiamine Vectors

The pyrithiamine or pPTR vectors are E. coli-Aspergillus shuttle vectors and contain an ampicillin resistance gene (AmpR) as the selection marker for E. coli and a pyrithiamine-resistance gene (ptrA) as the selection marker for Aspergillus. Pyrithiamine is a pyridine analog and inhibitor of thiamine. Because the pPTR vector multiple cloning site (MCS) is located within the lacZ region, the presence or absence of target DNA insert can easily be verified using blue/white screening via bacterial plates containing IPTG and X-Gal.

  At-A-Glance   Documents   Images & Data

Features

  • pPTR I DNA (4,782 bp) – Chromosomal integration vector for Aspergillus sp.
  • pPTR II DNA (10,032 bp) – Autonomously replicating vector for Aspergillus sp.

Applications

  • Selection of Aspergillus sp. transformants using pyrithiamine.

Notes

Because traces of thiamine decrease the bacterial selection efficiency of pyrithiamine media, reagents containing no thiamine should be used during transformation.

Manufactured by Hakutsuru Sake Brewing Co., Ltd.

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

References

1. Kubodera, T. et al. (2000) Biosci. Biotechnol. Biochem. 64:1416-1426.

Unique Restriction Sites

  • 3621: Hind III, Pst I, Sma I and Kpn I
  • 3622: Hind III, Sma I and Kpn I



 
 
Products
Cat. # Product Contents Size Price Units Select
3621 pPTR I DNA 20 ug $300.00
3622 pPTR II DNA 20 ug $300.00
 

 

 
 

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