PRIMER DESIGN TOOL
Our NEW In-Fusion Cloning Primer Design Tool allows for single- or multiple-insert cloning, accommodates vector linearization by inverse PCR or restriction digest, and enables site-directed mutagenesis. Simply input the DNA sequences of your vector and insert(s), along with your linearization method to generate primers for your next cloning experiment. Easily switch to the mutagenesis option to generate primers for all of your insertion, replacement, and deletion projects.
Update in progress...
CLASSIC PRIMER DESIGN TOOL
Design primers for In-Fusion single-insert PCR cloning. Intended for existing users (new users should try out the new tool).
MOLAR RATIO CALCULATOR
Calculate the optimal amounts of vector and insert for an In-Fusion Cloning reaction. Just enter the sizes of both vector and insert, in base pairs.
SIMULATE YOUR CONSTRUCT
SnapGene software allows easy planning and visualization of molecular biology procedures, including In-Fusion Cloning.