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Products >  Cloning_and_Competent_Cells >  Cloning_Kits >  In-Fusion_EcoDry

Cloning Kits (Lyophilized)—In-Fusion HD Plus EcoDry Cloning System

In-Fusion HD EcoDry Cloning Plus kits are provided in a convenient lyophilized, ready-to-use format that can be stored at room temperature. All the components for the cloning reaction are pre-aliquoted and dried down in tubes that are covered with an aluminum seal. Minimal handling eliminates chances of contamination, pipetting errors, or missing reagents. In-Fusion EcoDry Cloning Plus Systems allow you to clone any PCR fragment into any linearized vector in a single step without restriction digestion of the PCR fragment, ligation, or blunt-end polishing. The ability to clone directly into any vector at any restriction site eliminates the need for any further subcloning or manipulation.

How it Works

The In-Fusion Enzyme premix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15 bp overlap at their ends. This 15 bp overlap can be engineered by designing custom primers for amplification of the desired sequences. This method can be used to clone single or multiple fragments into a single vector without subcloning. All that’s required is a simple 30-minute incubation.

In-Fusion HD Plus EcoDry Cloning Kit Components

All In-Fusion HD Plus EcoDry Cloning kits contain the In-Fusion HD enzyme premix and reagents for control experiments, in a lyophilized format that provides ease of use and consistency. The In-Fusion premix comes in ready-to-use microtubes that can be stored right on the lab bench until you're ready to perform the In-Fusion reaction. In-Fusion HD Plus EcoDry Cloning kits also include:

  • CloneAmp HiFi High Fidelity PCR Premix,  a convenient 2X master mix that provides exceptionally accurate, efficient and fast DNA amplification. The premix contains dNTPs and an optimized buffer, allows rapid set-up of PCR reactions, and facilitates successful cloning.
  • Stellar Competent Cells, an HST08 E. coli strain that provides high transformation efficiency (greater than 5 x 108 cfu/µg) and compliments the efficiency of the In-Fusion HD Cloning Plus kit.
  • NucleoSpin Gel and PCR Clean-Up, for PCR purification and gel extraction.

  At-A-Glance   Documents   Images & Data   Resources

Features

In-Fusion HD EcoDry Cloning Plus systems includes all the reagents you need—including the PCR enzyme, competent cells, and gel extraction kit.

  • Ease of use and consistency—Due to lyophilized (EcoDry) format
  • Sub-cloning is unnecessary—Clone any insert, into any location, within any vector
  • Highly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes from 0.5 kb to 15 kb
  • Seamless construction—Final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
  • Flexibility to clone single or multiple fragments—Clone single or multiple DNA fragments simultaneously, in a single reaction
  • Can be used to perform site-directed mutagenesisClick here to learn how

Applications

  • Directional cDNA cloning
  • Multiple fragment cloning
  • Mutation generation
  • Gene synthesis
  • Adding adaptors, linker and protein tags
  • Cloning using modular parts
  • High-throughput cloning

Choose the Right In-Fusion Cloning Plus System for Your Needs

Lyophilized Format

Product Name
Cat. No.
Size
Purification Kit Included
Stellar Competent Cells Included
CloneAmp HiFi PCR Premix Included
In-Fusion HD EcoDry Cloning Plus
638912
8 rxns
Spin Columns
Yes
Yes
638913
24 rxns
Spin Columns
Yes
Yes
638914
48 rxns
Spin Columns
Yes
Yes
638915
96 rxns
Spin Columns
Yes
Yes

Liquid Format

Product Name
Cat. No.
Size
Purification Kit Included
Stellar Competent Cells Included
CloneAmp HiFi PCR Premix Included
In-Fusion HD Cloning Plus
638909
10 rxns
Spin Columns
Yes
Yes
638910
50 rxns
Spin Columns
Yes
Yes
638911
100 rxns
Spin Columns
Yes
Yes
638920
96 rxns
Spin Columns
Yes
Yes
In-Fusion HD Cloning Plus CE
638916
10 rxns
Cloning Enhancer
Yes
Yes
638917
50 rxns
Cloning Enhancer
Yes
Yes
638918
100 rxns
Cloning Enhancer
Yes
Yes
638919
96rxns
Cloning Enhancer
Yes
Yes

 

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
638912 In-Fusion® HD EcoDry™ Cloning Plus 8 Rxns $193.00 License Statements
638913 In-Fusion® HD EcoDry™ Cloning Plus 24 Rxns $477.00 License Statements
638914 In-Fusion® HD EcoDry™ Cloning Plus 48 Rxns $942.00 License Statements
638915 In-Fusion® HD EcoDry™ Cloning Plus 96 Rxns $1,365.00 License Statements
 

 

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Cat. # Product Contents Size Price Units Select
639298 CloneAmp™ HiFi PCR Premix 200 Rxns $180.00
 
 
 

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