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PCR Cloning Kit—Seamless DNA Cloning with In-Fusion HD Cloning Plus

In-Fusion PCR Cloning Kits enable directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The efficiency of In-Fusion HD cloning kits is over 95%.

How In-Fusion PCR Cloning Works

The In-Fusion Enzyme premix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15 bp overlap at their ends. This 15 bp overlap can be engineered by designing custom primers for amplification of the desired sequences. This method can be used to clone single or multiple fragments into a single vector without subcloning.

In-Fusion HD Cloning Plus System Components

All In-Fusion HD Cloning Plus kits contain the In-Fusion HD enzyme premix and reagents for control experiments. In-Fusion HD Cloning Plus kits also include:

  • CloneAmp HiFi High Fidelity PCR Premix
    CloneAmp HiFi PCR Premix is a convenient 2X master mix that provides exceptionally accurate, efficient and fast DNA amplification. The premix contains dNTPs and an optimized buffer, allows rapid set-up of PCR reactions, and facilitates successful cloning. 
  • Stellar Competent Cells
    An E. coli HST08 strain is included that provides high transformation efficiency (greater than 5 x 108 cfu/µg) and compliments the efficiency of the In-Fusion HD Cloning Plus kit.
  • Cloning Enhancer or NucleoSpin Gel and PCR Clean-Up
    • Cloning Enhancer (CE) is an easy-to-use enzyme premix that removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning. In-Fusion HD Cloning Plus CE kits are ideal for cloning when there is a single PCR product on the gel. 
    • Alternatively, NucleoSpin Gel and PCR Clean-Up is suitable for gel extraction as well as PCR purification.

At-A-Glance Documents Images & Data Resources

Features

In-Fusion HD Plus is a complete cloning system that includes all the reagents you need—including the PCR enzyme, competent cells, and PCR clean-up or gel extraction kit.

  • Sub-cloning is unnecessary—Clone any insert, into any location, within any vector
  • Highly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes from 0.5 kb to 15 kb
  • Seamless construction—Final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
  • Flexibility to clone single or multiple fragments—Clone single or multiple DNA fragments simultaneously, in a single reaction
  • Can be used to perform site-directed mutagenesisClick here to learn how

Applications

  • PCR cloning
  • HTP cloning
  • Multiple fragment cloning
  • Gene synthesis
  • Gene design
  • Mutagenesis
  • Domain swapping
  • Domain modification

Choose the Right In-Fusion Cloning Plus System for Your Needs

Liquid Format

Product Name
Cat. No.
Size
Purification Kit Included
Stellar Competent Cells Included
CloneAmp HiFi PCR Premix Included
In-Fusion HD Cloning Plus
638909
10 rxns
Spin Columns
Yes
Yes
638910
50 rxns
Spin Columns
Yes
Yes
638911
100 rxns
Spin Columns
Yes
Yes
638920
96 rxns
Spin Columns
Yes
Yes
In-Fusion HD Cloning Plus CE
638916
10 rxns
Cloning Enhancer
Yes
Yes
638917
50 rxns
Cloning Enhancer
Yes
Yes
638918
100 rxns
Cloning Enhancer
Yes
Yes
638919
96rxns
Cloning Enhancer
Yes
Yes

Lyophilized Format

Product Name
Cat. No.
Size
Purification Kit Included
Stellar Competent Cells Included
CloneAmp HiFi PCR Premix Included
In-Fusion HD EcoDry Cloning Plus
638912
8 rxns
Spin Columns
Yes
Yes
638913
24 rxns
Spin Columns
Yes
Yes
638914
48 rxns
Spin Columns
Yes
Yes
638915
96 rxns
Spin Columns
Yes
Yes

 

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
638909 In-Fusion® HD Cloning Plus 10 Rxns $212.00 License Statements
638910 In-Fusion® HD Cloning Plus 50 Rxns $824.00 License Statements
638911 In-Fusion® HD Cloning Plus 100 Rxns $1,426.00 License Statements
638920 In-Fusion® HD Cloning Plus 96 Rxns $1,373.00 License Statements
638916 In-Fusion® HD Cloning Plus CE 10 Rxns $219.00 License Statements
638917 In-Fusion® HD Cloning Plus CE 50 Rxns $875.00 License Statements
638918 In-Fusion® HD Cloning Plus CE 100 Rxns $1,477.00 License Statements
638919 In-Fusion® HD Cloning Plus CE 96 Rxns $1,410.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
639298 CloneAmp™ HiFi PCR Premix 200 Rxns $180.00
 
 

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