RNAiMonitor—RNA Interference Assay

This RNA intereference assay is designed to monitor knockdown of RNAi target sequences efficiently and easily, using a no-cell-lysis protocol. It consists of the pRNAiMonitor-MetLuc2 Vector and the Ready-To-Glow Secreted Luciferase Reporter Assay. The pRNAiMonitor-MetLuc2 vector expresses MetLuc, a secreted luciferase reporter that can easily be detected in the medium surrounding the cells. When a target sequence (encoding the target of your shRNA or siRNA) is cloned downstream of the MetLuc gene, a bicistronic mRNA transcript encoding both the MetLuc and the target sequence is produced. Target gene knockdown results in the degradation (or translational inhibition) of the mRNA transcript, which causes a proportional decrease in the expression of the reporter protein. The reporter molecule is easily detected with the Ready-To-Glow Secreted Luciferase Reporter Assay kits.

At-A-Glance

Documents

Images & Data

Resources

Features

RNA interference assay uses luciferase to monitor efficacy of shRNA or siRNA knockdown
Easy, no-cell-lysis protocol
Reporter vector for cloning target sequence

Products

Cat. #	Product	Contents	Size	Price	License	Units	Select

631755	RNAiMonitor		Each	$337.00

Required Products

Cat. #	Product	Contents	Size	Price	# of Units	Select

631727	Ready-To-Glow™ Secreted Luciferase Reporter Assay		500 Rxns	$367.00

631726	Ready-To-Glow™ Secreted Luciferase Reporter Assay		100 Rxns	$105.00

631728	Ready-To-Glow™ Secreted Luciferase Reporter Assay		1,000 Rxns	$518.00

RNAiMonitor—RNA Interference Assay

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Additional Information


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