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Products >  Cell_Biology_and_Epigenetics >  Pluripotency_Kits >  iPS_Efficiency_Check

iPS Efficiency Check qPCR Kit and Primer Set

The iPS Efficiency Check qPCR Kit lets you easily quantitate expression levels of key senescence markers to help you determine somatic cell reprogrammability, senescence marker expression levels (before and during reprogramming), and whether siRNA or knockdown construct(s) are required to enhance the efficiency of induced pluripotent stem (iPS) cell generation.

The kit provides all of the reagents needed to assess your target cell’s reprogrammability. It features prevalidated, forward and reverse primer mixes specific for four key senescence markers and one housekeeping (control) gene (Table I.) It also includes all of the reagents necessary for RNA extraction, first-strand cDNA synthesis, and quantitative real-time PCR. We also offer the iPS Efficiency Check qPCR Primer Set—which simply contains the prevalidated, forward and reverse primer mixes listed in Table I—for easy integration into your lab's existing workflow.

Table I. iPS Efficiency Check qPCR Primers

Primer SetAmplicon Size
Arf (senescence marker)67 bp
Ink4a (senescence marker)59 bp
p21 (senescence marker)140 bp
p53 (senescence marker)109 bp
Beta-Actin (control)151 bp

At-A-Glance Documents Images & Data

Features

  • Prevalidated qPCR primer mixes specific for key senescence markers
  • Complete kit to verify iPS generation: includes RNA extraction, reverse transcription, and qPCR reagents

Applications

  • Determine the reprogrammability of somatic cells before and during cell reprogramming
  • Measure senescence markers Arf, Ink4a, p21 and p53

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
632009 iPS Efficiency Check qPCR Kit Each $595.00 License Statements
632010 iPS Efficiency Check qPCR Primer Set 50 Rxns $350.00  
 

 

 


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