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Products >  Cell_Biology_and_Epigenetics >  Pluripotency_Kits >  Pluripotency_Check

Stem Cell Pluripotency Status Check Kit with PCR Primers

The Pluripotency Check PCR Kit contains all the necessary reagents to confirm that your mouse stem or iPS cells are indeed pluripotent. It includes 16 prevalidated forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes. The kit includes everything for start-to-finish confirmation of your cells’ pluripotency status, using any combination of primer sets, or the entire panel.

The Pluripotency Check PCR Primer Set consists of 16 prevalidated primer mixes based on nine genes expressed in pluripotent stem cells, plus two control genes. The different amplicon sizes allow you to distinguish between engogenous and exogenous expression.

Pluripotency Check PCR Primers

Primer setAmplicon sizePrimer setAmplicon size
Oct3/4 (Total)485 bpKlf4739 bp
Oct3/4 (Endogenous)224 bp Klf4 (Endogenous)206 bp
Nanog (Total)364 bp Ecat1164 bp
Nanog (Endogenous)223 bp ERas1210 bp
Sox2 (Total)193 bp Esg1376 bp
Sox2 (Endogenous)297 bp Rex1287 bp
c-Myc (Total)228 bp Beta-Actin (Control)603 bp
c-Myc (Endogenous)170 bp GAPDH (Control)568 bp

At-A-Glance Documents Images & Data

Features

Validate the pluripotent status of your stem cell or iPS cell culture using a simple PCR kit

  • 16 prevalidated PCR primer mixes
  • Complete kit with RNA extraction, reverse transcription, and PCR reagents
  • Confirm your iPS cells by differentiating between endogenous and exogenous gene expression

Applications

  • Validating mouse embryonic stem cell status
  • Confirming iPS status

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
631965 Pluripotency Check PCR Kit Each $650.00 License Statements
631966 Pluripotency Check PCR Primer Set 16 x 25 Rxns $350.00  
 

 

 


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