TaKaRa POD Conjugates are designed to replace traditional secondary antibodies in immunohistochemical staining of paraffin embedded tissue sections. Use these products for a simpler and easier method than those based on streptavidin-biotin and obtain clear, highly sensitive results with minimal background.
Each TaKaRa POD Conjugate consists of an amino acid polymer combined with peroxidase and the Fab' fragments of a secondary antibody. The labeled polymers bind to the primary antibody-antigen to form an HRP-labeled complex. The complex is visualized by the addition of a colorimetric substrate, such as DAB Substrate (see below).
This product is available in two different formats, for mouse tissue sections ("For Mouse Tissue") and for tissue sections from other mammalian sources ("For Tissue"). Specific versions are available based on the source species of the primary antibody; "Anti Mouse", "Anti Rat", and "Anti Rabbit" versions are available for mouse tissue sections, and "Anti Mouse", "Anti Rabbit", and "Anti Goat" versions are available for mammalian tissue.
DAB Substrate for HRP Detection
TaKaRa DAB Substrate includes reagents needed to prepare DAB (3,3'–diaminobenzidine tetrahydrochloride salt), a substrate that allows detection of horseradish peroxidase (HRP) activity. When DAB reacts with HRP, a dark brown-colored product forms, allowing visualization of peroxidase-conjugated antibodies bound to a sample. DAB is used in immunohistochemical staining and can also be used to stain other materials, including fixed cells and nitrocellulose membranes (Western blotting).