Clontech
login | register | order now | view cart | feedback
Products
Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Miscellaneous >  Reagents_and_Kits >  POD_Conjugate_DAB_Substrate

POD Conjugate for Immunohistochemical Staining of Paraffin Embedded Tissue Sections

TaKaRa POD Conjugates are designed to replace traditional secondary antibodies in immunohistochemical staining of paraffin embedded tissue sections. Use these products for a simpler and easier method than those based on streptavidin-biotin and obtain clear, highly sensitive results with minimal background.

Each TaKaRa POD Conjugate consists of an amino acid polymer combined with peroxidase and the Fab' fragments of a secondary antibody. The labeled polymers bind to the primary antibody-antigen to form an HRP-labeled complex. The complex is visualized by the addition of a colorimetric substrate, such as DAB Substrate (see below).

This product is available in two different formats, for mouse tissue sections ("For Mouse Tissue") and for tissue sections from other mammalian sources ("For Tissue"). Specific versions are available based on the source species of the primary antibody; "Anti Mouse", "Anti Rat", and "Anti Rabbit" versions are available for mouse tissue sections, and "Anti Mouse", "Anti Rabbit", and "Anti Goat" versions are available for mammalian tissue.

DAB Substrate for HRP Detection

TaKaRa DAB Substrate includes reagents needed to prepare DAB (3,3'–diaminobenzidine tetrahydrochloride salt), a substrate that allows detection of horseradish peroxidase (HRP) activity. When DAB reacts with HRP, a dark brown-colored product forms, allowing visualization of peroxidase-conjugated antibodies bound to a sample. DAB is used in immunohistochemical staining and can also be used to stain other materials, including fixed cells and nitrocellulose membranes (Western blotting).

  At-A-Glance   Documents   Images & Data

Features

TaKaRa POD Conjugates

  • Do not react with endogenous immunoglobulins (Ig)
  • Not affected by endogenous biotin
  • No need for a blocking reaction or addition of an enzyme-conjugated reagent that reacts with the secondary antibody

TaKaRa DAB Substrate

  • Easy-to-use—simply dilute the DAB solution in the provided buffer
  • Complete kit—solutions, tube for mixing, and convenient dropper bottle are included
  • Reliable results—clear staining results can be obtained

Applications

TaKaRa POD Conjugates

  • Immunohistochemical staining of paraffin embedded tissue sections
  • Antibody detection without the use of an enzyme-conjugated secondary

TaKaRa DAB Substrate

  • Detection of HRP-conjugated secondary antibodies used in immunohistochemical staining or Western blotting

Components

TaKaRa POD Conjugates

Cat. No. Volume
(for 60 rxns)
Target TissuePrimary Antibody Source
MK2016 mLMouseRat
MK2026 mLMouseRabbit
Guinea Pig
MK2036 mLOther MammalGoat
MK2046 mLOther MammalMouse
MK2056 mLOther MammalRabbit
Guinea Pig

TaKaRa DAB Substrate; Cat. # MK210

Reagent A (DAB color reagent)2.4 mL x 1
Reagent B (Substrate buffer)30  mL x 2
Tube with graduations 1
Tip (for Reagent B) 1
Dropper bottle    1

Storage

4°C

NOTE: Protect POD conjugates from light.

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
MK200 TaKaRa® POD Conjugate Set Anti Mouse, For Mouse Tissue 60 Assays $206.00
MK201 TaKaRa® POD Conjugate Anti Rat, For Mouse Tissue 6 mL $111.00
MK202 TaKaRa® POD Conjugate Anti Rabbit, For Mouse Tissue 6 mL $111.00
MK203 TaKaRa® POD Conjugate Anti Goat, For Tissue 6 mL $111.00
MK204 TaKaRa® POD Conjugate Anti Mouse, For Tissue 6 mL $111.00
MK205 TaKaRa® POD Conjugate Anti Rabbit, For Tissue 6 mL $111.00
MK210 TaKaRa® DAB Substrate 500 Rxns $275.00
 

 

 
 

Takara Bio USA is a Takara Bio Company © 2017 Privacy Policy | Terms & Conditions | Terms of Use