Rapid exosome isolation from cell culture media with Capturem technology
The importance of exosomes (small extracellular vesicles produced by most cell types) in the pathology of key diseases such as cancer have made them popular targets in both basic and pharmaceutical research. The Capturem Exosome Isolation Kit (Cell Culture) combines Capturem technology with a proprietary exosome-binding compound that is not antibody-based, allowing the isolation of exosomes from cell culture media in under 30 minutes. The kit provides preclearing columns to remove large cellular debris and isolation columns to specifically isolate exosomes, both of which are used with a standard benchtop centrifuge, eliminating the need for ultracentrifugation. These maxiprep-sized columns allow the processing of 25 ml of cell culture supernatant at a time. Additionally, the kit contains wash and elution buffers that have been optimized for the isolation protocol.