| Product | Comments |
|---|
| Systems for Promoter Studies (vectors included) |
|---|
| CherryPicker Cell Capture System, Cat. No. 632572 Contains the CherryPicker Reagent Kit, magnetic beads, a promoterless CherryPicker vector, and a control vector | Promoterless system suitable for use with most cell types. Use the CherryPicker Cell Capture System to capture and enrich cells in which your promoter of interest is active. |
| Lenti-X CherryPicker Cell Capture System, Cat. No. 632574 Contains the CherryPicker Reagent Kit, magnetic beads, a promoterless lentiviral CherryPicker vector, and a lentiviral control vector | Promoterless system which can be used to transduce virtually any dividing or nondividing cells, including primary cells and stem cells. Use the lentiviral CherryPicker Cell Capture System to capture and enrich cells in which your promoter of interest is active. |
| Systems for Protein Studies (vectors included) |
|---|
| CherryPicker Cell Capture System (IRES), Cat. No. 632573 Contains the CherryPicker Reagent Kit, magnetic beads, a bicistronic CherryPicker vector, and a control vector | Bicistronic system suitable for use with most cell types. Use the CherryPicker Cell Capture System (IRES) to capture and enrich cells expressing your protein of interest. |
| Lenti-X CherryPicker Cell Capture System (IRES), Cat. No. 632575 Contains the CherryPicker Reagent Kit, magnetic beads, a bicistronic lentiviral CherryPicker vector, and a lentiviral control vector | Bicistronic system which can be used to transduce virtually any dividing or nondividing cells, including primary cells and stem cells. Use the CherryPicker Cell Capture System (IRES) to capture and enrich cells expressing your protein of interest. |
| CherryPicker Reagents (no vectors included) |
|---|
| CherryPicker Assay Kit, Cat. Nos. 632570 & 632571 Contains the CherryPicker Reagent Kit (wash buffer & CherryPicker-specific antibody) and magnetic beads | Refill reagents for use with any CherryPicker System listed above. |
Capture Only Those Cells in which Your Promoter of Interest is Active
Use the promoterless CherryPicker systems, CherryPicker Cell Capture System (Cat. No. 632572) and Lenti-X CherryPicker Cell Capture System (Cat. No. 632574), to monitor the activation of a promoter of interest, and capture those cells containing the active promoter. Insertion of a functional promoter into the promoterless reporter vector, pCherryPicker1, causes a red fluorescent protein (CherryPicker, a membrane-targeted version of the mCherry fluorescent protein) to be displayed on the surface of mammalian cells, which are then easily captured by a CherryPicker-specific antibody bound to IgG-coated magnetic beads.
Capture Only Those Cells Expressing Your Protein
Use the bicistronic CherryPicker systems, CherryPicker Cell Capture System (IRES) (Cat. No. 632573) and Lenti-X CherryPicker Cell Capture System (IRES) (Cat. No. 632575) to identify, monitor, and capture mammalian cells expressing your protein of interest. IRES technology results in simultaneous expression of a protein of interest and a membrane-targeted red fluorescent protein (CherryPicker) from the same transcript. Cells expressing your protein of interest must also express CherryPicker, a membrane-targeted version of the mCherry fluorescent protein, and can be captured on magnetic beads via a CherryPicker-specific antibody.
Coexpression of your protein of interest and CherryPicker allows you to easily:
- Monitor cells expressing the protein of interest
- Capture and analyze cells expressing the protein of interest
- Culture the captured cells as a homogeneous population
