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–20°C (Cat. # MK426 and MK429)
Adipocyte Differentiation: Adipocyte Differentiation Inducer and GAPDH Assay Kit
Cat. # MK429
Kit contains 3 sets of reagents (for 100 ml differentiation medium + 200 ml maintenance medium)
|Additive reagents for differentiation medium|
|Insulin solution (final conc. 10 µg/ml)*||1 ml x 3|
|Dexamethasone solution (final conc. 2.5 µM)*||0.5 ml|
|3-isobutyl-1-methylxanthine solution (final conc. 0.5 mM)*||0.1 ml|
|Additive reagent for maintenance medium|
|Insulin solution (final conc. 10 µg/ml)*||2 x 1 ml|
* Final concentration after the specified volume is added to the medium
Cat. # MK426
|Microtiter Plate||1 plate (96 wells) |
|GPDH Substrate||1 x 11 ml |
|Enzyme Extraction Buffer||1 x 11 ml |
|Dilution Buffer ||2 x 11 ml |
Cat. # MK426
- GPDH activity measurement
- Adipocyte differentiation
- Differentiation repressor screening
Cat. # MK426
- Assay duration: 2–10 minutes (following sample addition)
- Sample volume per well: 25 µL
Cat. # MK429
- Contains three commonly used adipocyte differentiation-inducers: insulin, 3-isobutyl-1-methylxanthine, and dexamethasone
- Efficiently induces adipocyte differentiation in mouse embryonic fibroblast 3T3-L1 cells, rat brown/white preadipocytes, mouse/rat/rabbit bone marrow cells, and other animal cells
Adipocyte differentiation is an important research area since adipose tissue plays an essential role in energy homeostasis. Adipocyte differentiation regulation by transcriptional and epigenetic mechanisms is also a popular field of investigation. Mouse embryonic fibroblast 3T3-L1 cells (ATCC No. CL-173) are one of the most commonly used cell models in the study of adipocyte differentiation because they easily differentiate into adipocytes when induced by insulin, 3-isobutyl-1-methylxanthine, and dexamethasone.
The enzyme glycerol 3-phosphate dehydrogenase (GPDH) catalyzes a reversible reaction between dihydroxyacetone phosphate (DHAP) and glycerol 3-phosphate. NAD is utilized as a coenzyme. GPDH activity increases significantly during adipocyte differentiation; as a result, GPDH activity measurement is used to monitor fat synthesis.
Adipoinducer Reagent for Mammalian Cells
The Adipoinducer Reagent for Mammalian Cells efficiently induces adipocyte differentiation in mouse embryonic fibroblast 3T3-L1 cells, rat brown/white preadipocytes, mouse/rat/rabbit bone marrow cells, and other animal cells. This kit contains three of the most frequently used inducers of adipocyte differentiation: insulin, 3-isobutyl-1-methylxanthine, and dexamethasone. Adipocyte differentiation can be achieved by culturing cells in an appropriate medium containing the aforementioned inducer agents.
GPDH Activity Assay Kit
The Glycerol 3-Phosphate Dehydrogenase or GPDH Activity Assay Kit is a 96-well format, microtiter plate assay designed to measure the activity of GPDH. This kit can be used to study mechanisms that regulate adipocyte differentiation wherein primary or established progenitor cells differentiate into adipocytes. The assay procedure involves cell extract generation via the addition of the Enzyme Extraction Buffer followed by serial dilution of the extract. The serially diluted extract is then added to a 96-well plate containing GDPH Substrate solution and GPDH activity is calculated by measuring the change (decrease) in OD340 absorbance using a microtiter plate reader.