The MDM4 gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus. It is the human homolog of mouse double minute 4 (MDM4) protein and shows structural similarity to p53-binding protein MDM2. MDM4 is also called Mdm4 p53 binding protein homolog (mouse); transformed 3T3 cell double minute 4, p53 binding protein (mouse); MDMX, Mdm2-like p53-binding protein, HDMX, MRP1, MDM4-related protein 1, double minute 4 protein, and p53-binding protein Mdm4. Both MDM4 and MDM2 bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, MDM4 inhibits p53 by binding its transcription activation domain. MDM4 can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 activity.
This product is an affinity-purified IgG antibody that recognizes human MDM4/MDMX protein. The antibody was raised in mouse using recombinant MDM4/MDMX protein, and can be used for Western blot (WB) detection of human MDM4/MDMX protein.