Interferon induced with helicase C domain 1 (IFIH1) protein is a member of a group of RNA helicases, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), known as DEAD box proteins. IFIH1 is also known as melanoma differentiation-associated protein 5 (MDA5), clinically amyopathic dermatomyositis autoantigen 140 kDa, helicase with 2 CARD domains (HLCD), helicard, murabutide down-regulated protein, RIG-I-like receptor 2 (RLR-2), CADM-140 autoantigen, RNA helicase-DEAD box protein 116 (RH116), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, and IDDM19. IFIH1/MDA5 plays a major role in sensing viral infection and in the activation of a cascade of antiviral and inflammatory responses. IFIH1 gene expression is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Treatment with both these agents causes irreversible reprogramming of melanomas, while treatment with either agent alone only achieves reversible differentiation. Mutations in the IFIH1 gene are associated with diabetes mellitus insulin-dependent, type 19.
This product is an affinity-purified IgG antibody that recognizes human IFIH1/MDA5 protein. The antibody was raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection of human IFIH1/MDA5 protein.