- Western blot analysis under reducing and non-reducing conditions.
Human Ago2 N-terminal monoclonal antibody was obtained by fusing the the mouse myeloma cell line P3U1 with lymph cells of BALB/c mouse lymph cells following immunization with the N-terminal peptide (1 - 148) of recombinant human Argonaute 2 (Ago2) protein (NP_036286.2). The Argonaute 2 antibody was purified from mouse ascitic fluid.
This product does not contain preservative.
The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for up to 1 year or at 4°C for up to 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw. Diluted antibody should not be stored.
|hAgo2 1B1||Human Ago2 protein||Mouse IgG2a (lambda)|
Dissolve the lyophilized antibody in 100 μl of distilled water (final concentration: 2.0 mg/ml). This solution can be used as a stock solution. If further dilution is required, use the noted dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μl or more of the noted dilution solution.
Note (1) : Be sure to store the antibody at a minimum concentration of 2.0 mg/ml. A lower antibody concentration may result in decreased stability.
Note (2) : Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
10 mM PBS (pH7.4)
* When stored at 4°C, 0.1% sodium azide should be added as a preservative.
1) Azuma-Mukai, A. et al. (2008) Proc. Natl. Acad. Sci. USA 5(23):7964–7969.