Human primary hepatocytes (hphep) are an invaluable tool for academic and pharmaceutical labs studying liver disease pathology or as part of the drug development pipeline. To assess disease models and hepatocyte toxicity, robust and predictive hphep cell cultures are required. However, currently available culture media severely limits the utility of primary hepatocytes because hepatocytes lose their function and viability within a week. Additionally, daily feedings are required to maintain the hphep cultures.
Cellartis Power Primary HEP Medium is specially formulated to enable the long-term maintenance—cells are viable and maintain functionality for four weeks—of cryopreserved plateable hphep in conventional 2D cultures. This medium further simplifies culturing of hepatocytes with a weekend-free feeding schedule.