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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Metabolic_Diseases >  Lipoprotein_Lipase

Performance

  • Range of assay: 0.04 - 2.8 ng/mL
  • Assay sensitivity: 0.009 ng/mL
  • Assay duration: Approximately 2-2.5 hours
  • Test specimen type: EDTA-plasma, serum, cell culture supernatants
  • Sample volume per well: 100 µL

Lipoprotein Lipase Assay

Storage

2–8°C
Lipoprotein Lipase | Lipoprotein Lipase assay | LPL ELISA

Alternative Names

LPL, LIPD, EC 3.1.1.34, HDLCQ11

Components

Precoated plate: Anti-Human LPL (57A5) Mouse
IgG MoAb Affinity Purify
96 Well x 1
Labeled antibody: (30X) HRP conjugated Anti- Human LPL (88B8)
Mouse IgG MoAb Affinity Purify
0.4 mL x 1
Standard: Recombinant human LPL1.0 mL x 2
EIA buffer30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): Phosphate buffer50 mL x 1

Applications

  • Measuring human LPL in EDTA-plasma, serum, or cell culture supernatant samples

Features

  • Capable of measuring lipoprotein lipase (LPL)
  • Sensitivity: 0.009 ng/mL

This kit is a solid phase sandwich ELISA that uses two highly specific antibodies to detect human lipoprotein lipase (LPL); one antibody is precoated on the ELISA plate, and the other is HRP-conjugated. This kit can be used to quantify human LPL in EDTA-plasma, serum, or cell culture supernatant samples.

Lipoprotein lipase (LPL) is involved in hydrolysis of triglyceride-rich lipoproteins, including circulating chylomicrons (CMs) and very low-density lipoproteins (VLDLs). Secreted LPL binds to heparin sulfate proteoglycans at the surface of the capillary endothelium and hydrolyzes triglyceride (TG) into free fatty acids and monoglycerides. Deficiency or reduction in LPL activity is one cause of hypertriglyceridemia (increased levels of triglycerides). This ELISA kit can be used to measure small amounts of circulating LPL in pre-heparin blood samples or LPL levels in cell culture supernatants.

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