Leptin is an adipose-derived hormone that acts through a signaling pathway to regulate energy (food) intake and expenditure, maintaining steady levels of adipose tissue mass. Leptin was discovered in studies of obesity in mice. In humans, leptin is a 16-kDa protein, encoded by the LEP gene, and is expressed mostly in white adipose tissue. Leptin acts through the leptin receptor, which exists as multiple isoforms. In addition to its role in adipose tissue, leptin regulates immune and inflammatory responses, hematopoiesis, angiogenesis, and wound healing. Mutations in the LEP gene cause severe to morbid obesity and hypogonadism and are associated with the development of type 2 diabetes. Leptin is also known as obesity protein (OB), obesity factor, OBS, and LEPD.
ELISAs for Leptin Detection
These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to leptin; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human, mouse, or rat leptin protein in serum, EDTA plasma, or cell culture supernatants.
Antibodies for Leptin Detection
These products are affinity-purified IgG antibodies that recognize human, mouse, or rat leptin protein. The antibodies were raised in rabbit using synthetic peptides, and can be used for Western blot (WB) detection of leptin protein.