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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Metabolic_Diseases >  Leptin

Leptin Detection

Leptin is an adipose-derived hormone that acts through a signaling pathway to regulate energy (food) intake and expenditure, maintaining steady levels of adipose tissue mass. Leptin was discovered in studies of obesity in mice. In humans, leptin is a 16-kDa protein, encoded by the LEP gene, and is expressed mostly in white adipose tissue. Leptin acts through the leptin receptor, which exists as multiple isoforms. In addition to its role in adipose tissue, leptin regulates immune and inflammatory responses, hematopoiesis, angiogenesis, and wound healing. Mutations in the LEP gene cause severe to morbid obesity and hypogonadism and are associated with the development of type 2 diabetes. Leptin is also known as obesity protein (OB), obesity factor, OBS, and LEPD.

ELISAs for Leptin Detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to leptin; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human, mouse, or rat leptin protein in serum, EDTA plasma, or cell culture supernatants.

Antibodies for Leptin Detection

These products are affinity-purified IgG antibodies that recognize human, mouse, or rat leptin protein. The antibodies were raised in rabbit using synthetic peptides, and can be used for Western blot (WB) detection of leptin protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
 
Cross‑Reactivity
 
Leptin27775AELISAHuman

Capture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

Cross‑reactivity: mouse 2.4%, rat ≤0.1%

Leptin27160AELISAMouse

Capture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

Cross‑reactivity: rat 43.6%, human 0.3%

Leptin27295AELISARat

Capture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

Cross‑reactivity: mouse 17.9%, human 0.2%
Leptin (C‑terminus)18545AWBHuman(n/a)
Rabbit IgG

Cross-reacts with mouse and rat leptin

Leptin (C‑terminus)18546AWBRat(n/a)
Rabbit IgG
Cross-reacts with mouse leptin
Leptin (C‑terminus)18547AWBMouse(n/a)
Rabbit IgG
Cross-reacts with rat leptin

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
18545A Anti-Human Leptin (C) Rabbit IgG Affinity Purify 100 ug $499.00
18546A Anti-Rat Leptin (C) Rabbit IgG Affinity Purify 100 ug $499.00
18547A Anti-Mouse Leptin (C) Rabbit IgG Affinity Purify 100 ug $499.00
27775A Human Leptin (highly sensitive) Assay Kit - IBL 96-Well $606.00
27160A Mouse Leptin Assay Kit - IBL 96-Well $606.00
27295A Rat Leptin Assay Kit - IBL 96-Well $606.00
 

 

 
 

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