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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Metabolic_Diseases >  Insulin

Insulin Detection

Insulin is produced in a step-wise process. Preproinsulin is a biologically inactive precursor that consists of an N-terminal signal peptide followed by the proinsulin molecule. Removal of the signal peptide by signal peptidases results in proinsulin alone. Proinsulin contains the A-chain, B-chain, and a 31-amino-acid Connecting peptide or C-peptide. The C-peptide is positioned between the A-chain and the B-chain and functions to connect them. The C-peptide is removed once proinsulin is processed into insulin.

Antibodies for Insulin Detection

Human insulin monoclonal antibody may be used for the analysis of the structure, function, and metabolism of insulin. This anti-insulin antibody, also known as INS antibody, is applicable for Western blot (WB) analysis under non-reducing and non-heating conditions and for immunohistological (IHC) studies of frozen tissue sections.

Antibodies to the proinsulin C-peptide (also known as insulin C) are frequently used to study the structure, function, and metabolism of insulin as well as proinsulin. Insulin C antibodies can also be used for immunocytochemistry (ICC) and for immunohistochemical (IHC) studies of paraffin-embedded or frozen tissue sections. The following insulin C antibodies are available:

  • Human insulin C monoclonal antibody is generated in mouse immunized with the KLH-conjugated human insulin C-peptide (71–86) [GPGAGSLQPLALEGSL].
  • Mouse insulin C monoclonal antibody is generated in rat immunized with the KLH-conjugated mouse insulin C-peptide (71–84) [SPGDLQTLALEVAR].
  • Mouse insulin C polyclonal antibody is generated in guinea pig immunized with the KLH-conjugated mouse insulin C-peptide (71–84) [SPGDLQTLALEVAR].

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Features

Antigen
Cat. #
Application
Species
(Clone) and Source
 
Cross‑Reactivity
 
InsulinM056IHC
WB
Structural, functional, and metabolic analyses of proinsulin and insulin
Human

(IS11-1)
Mouse IgG1 MoAb

Cross‑reacts with bovine and porcine insulin

Insulin C
(71–86)
M232IHC
Structural, functional, and metabolic analyses of proinsulin and insulin
Human

(h-Ins 1B-1)
Mouse IgG2b 
MoAb

Does not cross‑react with human insulin A- and B-peptides, mouse C-peptide, or rat C‑peptide

Insulin C (71–84)M233IHC
Structural, functional, and metabolic analyses of proinsulin and insulin
Mouse

Mouse 
(M-Ins 1J-4)
Rat IgG2a MoAb

Does not cross-react with mouse insulin A- or B-peptides
Does not cross-react with human C-peptide or rat C-peptide
Insulin C (71–84)M178IHC
ICC
Structural, functional, and metabolic analyses of proinsulin and insulin
Mouse(n/a)
Guinea pig PoAb

Does not cross-react with human insulin C-peptide

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price Units Select
M232 Anti-Human Insulin C, Monoclonal (Clone h-Ins 1B-1) 0.1 mg $357.00
M233 Anti-Mouse Insulin C, Monoclonal (Clone M-Ins 1J-4) 0.1 mg $357.00
  M178 Anti-Mouse Insulin C, Polyclonal 0.1 mg $357.00
  M056 Monoclonal Anti-Human Insulin (Clone IS11-1) 0.2 mg $214.00
 

 

 
 

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