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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Metabolic_Diseases >  Glucagon

Glucagon Detection

Glucagon is a hormone consisting of 29 amino acids, with a molecular weight of approximately 3.5 kDa. It is synthesized in pancreatic alpha cells and is secreted after processing. It promotes an increase in blood sugar levels, a role that is in direct opposition to that of insulin. When the body falls into a low blood sugar state, glucagon acts in the liver, breaking down stored glycogen, transferring sugar into the bloodstream, and maintaining homeostasis of sugar concentration in the blood.

ELISAs for Glucagon Detection

The Human Glucagon EIA Kit (Cat. # MK156) is a colorimetric two-antibody sandwich-type EIA method designed to quantitatively measure human glucagon in serum, plasma, and cultured medium by recognizing the 29-amino acid intact glucagon domain, using antibodies against both the N- and C-terminal regions. Although glucagon is thought to have just small differences in amino acid sequences between animal species, this kit is optimized for the measurement of human antigens.

The Mouse/Rat/Pig Glucagon Competitive EIA Kit (Cat. # MK157) is a single antibody-antigen competitive EIA method designed to quantitatively measure glucagon in serum, plasma, culture medium, and other samples, using a rabbit polyclonal antibody that reacts with glucagon at a comprehensive species level. Although human antigens can also be measured using this kit, we recommend using the two-antibody sandwich-type assay described above (Human Glucagon EIA Kit; Cat. # MK156) for measurement of human samples.

Antibodies for Glucagon Detection

This glucagon antibody can be applied towards the immunohistochemical (IHC) detection of human glucagon in paraffin-embedded tissue and frozen tissue sections. The polyclonal anti-glucagon is raised in Guinea pig against a human glucagon immunogen.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
 
GlucagonMK156ELISAHuman

Capture:
(n/a)

Detect: Biotin
(n/a)

GlucagonMK157Competitive
ELISA
Mouse
Rat
Porcine

Capture:
(n/a)

Detect:
(n/a)
Rabbit PoAb

GlucagonM182IHCHuman

(n/a)
Guinea pig PoAb

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price Units Select
  M182 Polyclonal Anti-Glucagon 0.1 mg $334.00
MK156 Human Glucagon EIA Kit 96-Well $745.00
MK157 Mouse/Rat/Pig Glucagon Competitive EIA Kit 96-Well $705.00
 

 

 
 

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