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Cell Biology & Epigenetics

Products >  Cell_Biology_and_Epigenetics >  Metabolic_Diseases >  Gastric_Inhibitory_Peptide

Gastric Inhibitory Polypeptide (GIP) ELISA

The Gastric Inhibitory Polypeptide (GIP) ELISA kits are solid-phase ELISAs that use two highly specific GIP antibodies; one GIP antibody is precoated onto the ELISA plate and the other antibody is HRP-conjugated. These GIP assay kits can measure soluble active GIP (1-42) or total GIP (active (1-42) and inactive (3-42)) in EDTA-plasma. Please note that GIP (1-42) is rapidly inactivated by dipeptidyl peptidase IV (DPP-IV); therefore, samples should be treated with a DPP-IV inhibitor before performing assays for the active form.

Gastric inhibitory polypeptide (GIP), also known as glucose-dependent insulinotropic peptide, is a member of the incretin family of hormones. Following food ingestion, these gastrointestinal hormones promote insulin secretion from beta cells while inhibiting glucagon release from alpha cells within the islets of Langerhans. In addition to the pancreas, GIP receptor expression has been reported in adipocytes and osteoblasts. The regulation of GIP signaling pathways may lead to metabolic syndrome improvement. GIP is derived from a 153-amino acid precursor encoded by the GIP gene. The biologically active form of GIP is a 42-amino acid peptide termed GIP (1-42). GIP is rapidly inactivated to GIP (3-42) by blood DPP-IV.

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  • Capable of quantifying human GIP protein


  • For measurement of active GIP (1-42) protein from human, rat, or mouse EDTA-plasma.
  • For measurement of total GIP from human, rat, or mouse EDTA-plasma.


Precoated plate: Anti-GIP(C) Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-GIP (N) (6A1A) Mouse
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: GIP(1-42)0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB Solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1




Cat. #27201A27203A
Range of assay 1.6–100 pg/mL1.88 - 120 pmol/L
Assay sensitivity 1.2 pg/mL0.82 pmol/L
Specificity Active human GIPTotal human GIP
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: EDTA-plasma
  • Sample volume per well: 100 µL

Cat. # Product Contents Size Price Units Select
27201A Human GIP, Active form Assay Kit - IBL 96-Well $929.00
27203A Human GIP, Total Assay Kit - IBL 96-Well $1,017.00



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