The Gastric Inhibitory Polypeptide (GIP) ELISA kits are solid-phase ELISAs that use two highly specific GIP antibodies; one GIP antibody is precoated onto the ELISA plate and the other antibody is HRP-conjugated. These GIP assay kits can measure soluble active GIP (1-42) or total GIP (active (1-42) and inactive (3-42)) in EDTA-plasma. Please note that GIP (1-42) is rapidly inactivated by dipeptidyl peptidase IV (DPP-IV); therefore, samples should be treated with a DPP-IV inhibitor before performing assays for the active form.
Gastric inhibitory polypeptide (GIP), also known as glucose-dependent insulinotropic peptide, is a member of the incretin family of hormones. Following food ingestion, these gastrointestinal hormones promote insulin secretion from beta cells while inhibiting glucagon release from alpha cells within the islets of Langerhans. In addition to the pancreas, GIP receptor expression has been reported in adipocytes and osteoblasts. The regulation of GIP signaling pathways may lead to metabolic syndrome improvement. GIP is derived from a 153-amino acid precursor encoded by the GIP gene. The biologically active form of GIP is a 42-amino acid peptide termed GIP (1-42). GIP is rapidly inactivated to GIP (3-42) by blood DPP-IV.
