Glucagon is a hormone consisting of 29 amino acids, with a molecular weight of approximately 3.5 kDa. It is synthesized in pancreatic alpha cells and is secreted after processing. It promotes an increase in blood sugar levels, a role that is in direct opposition to that of insulin. When the body falls into a low blood sugar state, glucagon acts in the liver, breaking down stored glycogen, transferring sugar into the bloodstream, and maintaining homeostasis of sugar concentration in the blood.
ELISAs for Glucagon Detection
The Human Glucagon EIA Kit (Cat. # MK156) is a colorimetric two-antibody sandwich-type EIA method designed to quantitatively measure human glucagon in serum, plasma, and cultured medium by recognizing the 29-amino acid intact glucagon domain, using antibodies against both the N- and C-terminal regions. Although glucagon is thought to have just small differences in amino acid sequences between animal species, this kit is optimized for the measurement of human antigens.
The Mouse/Rat/Pig Glucagon Competitive EIA Kit (Cat. # MK157) is a single antibody-antigen competitive EIA method designed to quantitatively measure glucagon in serum, plasma, culture medium, and other samples, using a rabbit polyclonal antibody that reacts with glucagon at a comprehensive species level. Although human antigens can also be measured using this kit, we recommend using the two-antibody sandwich-type assay described above (Human Glucagon EIA Kit; Cat. # MK156) for measurement of human samples.
Antibodies for Glucagon Detection
This glucagon antibody can be applied towards the immunohistochemical (IHC) detection of human glucagon in paraffin-embedded tissue and frozen tissue sections. The polyclonal anti-glucagon is raised in Guinea pig against a human glucagon immunogen.