Endothelins are among the most potent vasoconstrictor proteins known. Overexpression of endothelins contributes to hypertension and heart disease. Three isoforms are known, each containing 21 amino acids: endothelin-1 (ET-1), endothelin-2 (ET-2), and endothelin-3 (ET-3). All three isoforms are encoded by a 38-amino-acid precursor known as big endothelin. Endothelins are expressed in many tissues, including lung, kidney, brain, pituitary, and placenta.
ELISAs for Big Endothelin-1 Detection
These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human or rat big ET-1 protein; one is precoated on the ELISA plate, and the other is HRP-conjugated. These kits can be used to measure soluble human or rat big ET-1 protein in serum, EDTA-plasma, cell culture supernatants, or tissue extracts.
Antibodies for Big Endothelin-1 Detection
These products are affinity-purified IgG antibodies that recognizes human and rat big ET-1. The antibodies were raised in rabbit using synthetic big ET-1 (22-38) peptides, and can be used for immunohistochemical (IHC) detection of human or rat big ET-1 protein.