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Products >  Cell_Biology_and_Epigenetics >  Metabolic_Diseases >  Angiotensinogen

Angiotensinogen Detection

Angiotensinogen (also known as serpin peptidase inhibitor, clade A, member 8; Serpin A8, Ang, Ang II, PAT, and ANRT) is a 452-amino acid hormone precursor produced by the liver and is implicated in blood pressure control. Urinary excretion of angiotensinogen in a rat hypertension model indicates that this protein is involved in the intrarenal renin-angiotensin system (RAS). In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-I, which is further cleaved by angiotensin-converting enzyme (ACE) to yield the physiologically active peptide angiotensin-II. Angiotensin-II stimulates aldosterone release, promoting sodium retention in the kidney and an increase in blood pressure. Angiotensin II is also implicated in the regulation of insulin-like growth factor 1 (IGF-1) expression.

ELISAs for Angiotensinogen Detection

These ELISA kits are solid-phase sandwich ELISAs that take advantage of two highly specific antibodies; one antibody is coated onto the ELISA plate and the other is HRP-conjugated.

  • The Human, Mouse, and Rat Total Angiotensinogen Assay Kits are ELISA kits that can be used to detect total or intact human, mouse or rat angiotensinogen in serum, EDTA-plasma, urine, or cell culture media.
  • The Mouse and Rat Intact Angiotensinogen Assay Kits are ELISA kits that can be used to detect C-terminal intact mouse or rat angiotensinogen in serum, EDTA-plasma, urine, or cell culture supernatant.

Antibodies for Angiotensinogen Detection

These affinity-purified antibodies recognize either mouse/rat or human angiotensinogen protein. The antibodies can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of angiotensinogen protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
 
Cross‑Reactivity
 
Angiotensinogen27412A, DELISAHuman

Capture:
(72)
Mouse IgG

Detect: HRP
(601)
Mouse IgG MoAb

Shows ≤0.1 cross‑reactivity against angiotensin proteins I–IV, angiotensin (1–7), angiotensin (1–9), human albumin, human IgG, and human angiopoietin-like 3
Angiotensinogen27413A, DELISAMouse

Capture:
(135)
Rabbit IgG

Detect: HRP
(405)
Rabbit IgG

Shows ≤0.1 cross-reactivity against angiotensin proteins I–IV, angiotensin (1–7), angiotensin (1–9), mouse albumin, mouse IgG, and mouse angiopoietin-like 3
Angiotensinogen27413D 27414A, DELISARatCapture:
(135)
Rabbit IgG

Detect: HRP
(405)
Rabbit IgG
Shows ≤0.1 cross-reactivity against angiotensin proteins I–IV, angiotensin (1–7), angiotensin (1–9), rat albumin, and rat transferrin
Angiotensinogen27743AELISAMouseCapture:
(A1)
Rabbit IgG

Detect: HRP
(405)
Rabbit IgG
Shows ≤0.1 cross-reactivity against angiotensin proteins I–IV, angiotensin (1–7), angiotensin (1–9), normal mouse IgG, mouse albumin, and mouse transferrin
Angiotensinogen27744AELISARatCapture:
(A1)
Rabbit IgG

Detect: HRP
(405)
Rabbit IgG
Shows ≤0.1 cross-reactivity against angiotensin proteins I–IV, angiotensin (1–7), angiotensin (1–9), normal rat IgG, rat albumin, and rat transferrin
Angiotensinogen (C-terminus; 394–485)10417A, BWBHuman(104AT 601.2.80)
Mouse IgG MoAb
n/a
Mouse angiotensinogen (C-terminus)28101A, BIHC
WB
Mouse
Rat
(405)
Rabbit IgG
n/a

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price Units Select
  27412A Human Total Angiotensinogen Assay Kit - IBL 96-Well $849.00
  27412D Human Angiotensinogen EIA buffer 100 100 mL $172.00
  27413A Mouse Total Angiotensinogen Assay Kit - IBL 96-Well $849.00
  27413D Mouse/Rat Angiotensinogen EIA buffer 100 100 mL $172.00
  27414A Rat Total Angiotensinogen Assay Kit - IBL 96-Well $849.00
  27414D Rat Total Angiotensinogen EIA Buffer 30 mL $75.00
  28101A Anti-Mouse/Rat Angiotensinogen (405) Rabbit IgG Affinity Purify 10 ug $113.00
  28101B Anti-Mouse/Rat Angiotensinogen (405) Rabbit IgG Affinity Purify 100 ug $499.00
  10417A Anti-Human Angiotensinogen (104AT 601.2.80) Mouse IgG MoAb 10 ug $114.00
  10417B Anti-Human Angiotensinogen (104AT 601.2.80) Mouse IgG MoAb 100 ug $525.00
27743A Mouse Intact Angiotensinogen Assay Kit - IBL 96-Well $924.00
27744A Rat Intact Angiotensinogen Assay Kit - IBL 96-Well $924.00
 

 

 
 

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