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Products >  Cell_Biology_and_Epigenetics >  Metabolic_Diseases >  Angiopoietin-Like_Protein

Angiopoietin-Like Protein Detection

Angiopoietin-like proteins are a family of secreted glycoproteins that have strong structural similarity to angiopoietins. They contain a signal peptide, N-terminal coiled-coil domain, and a C-terminal fibrinogen (FBN)-like domain. Angiopoietin-like protein 2 (ANGPTL2) is believed to induce sprouting in endothelial cells through autocrine and paracrine actions. It has been implicated in the metastasis of tumor cells in mouse models. ANGPTL2 is also known as angiopoietin-like 2, angiopoietin-related protein 2 (ARP2), and HARP.

The FBN domain in angiopoietin-like protein 3 (ANGPTL3) interacts with alpha-5 and beta-3 integrins, leading to induction of endothelial cell adhesion and migration. ANGPTL3 may play a role in angiogenesis. It is expressed primarily in the liver and positively correlates with high-density lipoprotein cholesterol (HDL-C) levels. Loss-of-function mutations in one copy of the ANGPTL3 gene are associated with lower levels of low-density lipoprotein cholesterol (LDL-C); in both copies, with lower levels of LDL-C, HDL-C, and triglycerides. ANGPTL3 is also known as angiopoietin-like 3, angiopoietin-5 (ANGPT5), ANG-5, and FHBL2.

Angiopoietin-like protein 4 (ANGPTL4) is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. It also functions as an apoptosis survival factor in vascular endothelial cells. ANGPTL4 is thought to have an antimetastatic function in several cancers; it has been shown to inhibit vascular activity as well as tumor cell motility and invasiveness. Reduced expression of the ANGPTL4 gene has been associated with type 2 diabetes. ANGPTL4 is also known as angiopoietin-like 4, angiopoietin-related protein 4 (ARP4), hepatic fibrinogen/angiopoietin-related protein (HFARP1), peroxisome proliferator-activated receptor (PPAR), PPAR gamma angiopoietin-related protein (PGAR), fasting-induced adipose factor (FIAF), NL2, and pp1158.

ELISAs for Angiopoietin-Like Protein Detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human or mouse ANGPTL protein variants; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human or mouse ANGPTL protein variants in serum, EDTA plasma, and cell culture supernatants.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
ANGPTL227745AELISAHuman

Capture:
(K2-1A1A)
Mouse IgG MoAb

Detect: HRP
(K1-12A4A)
Mouse IgG MoAb

Shows <0.1% cross‑reactivity with human ANGPTL 3, 4, and 6
ANGPTL327750AELISAHuman

Capture:
(45B1)
Mouse IgG MoAb

Detect: HRP
(F48A1)
Mouse IgG MoAb

Shows <0.1% cross-reactivity with human ANGPTL 2, 4, and 6
ANGPTL327410AELISAMouseCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
n/a
ANGPTL427749AELISAHumanCapture:
(K3-21A1A)
Mouse IgG MoAb

Detect: HRP
(K2-19A1A)
Mouse IgG MoAb
Shows <0.1% cross-reactivity with human ANGPTL2 and ANGPTL3
Note: Cell culture media containing FBS or FCS will give high ANGPTL4 readings
Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
ANGPTL227745AELISAHuman

Capture:
(K2-1A1A)
Mouse IgG MoAb

Detect: HRP
(K1-12A4A)
Mouse IgG MoAb

Shows <0.1% cross‑reactivity with human ANGPTL 3, 4, and 6
ANGPTL327750AELISAHuman

Capture:
(45B1)
Mouse IgG MoAb

Detect: HRP
(F48A1)
Mouse IgG MoAb

Shows <0.1% cross-reactivity with human ANGPTL 2, 4, and 6
ANGPTL327410AELISAMouseCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
n/a
ANGPTL427749AELISAHumanCapture:
(K3-21A1A)
Mouse IgG MoAb

Detect: HRP
(K2-19A1A)
Mouse IgG MoAb
Shows <0.1% cross-reactivity with human ANGPTL2 and ANGPTL3
Note: Cell culture media containing FBS or FCS will give high ANGPTL4 readings

 
 
Products
Cat. # Product Contents Size Price Units Select
27745A Human ANGPTL2 Assay Kit - IBL 96-Well $849.00
27410A Mouse angiopoietin-like 3 Assay Kit - IBL 96-Well $606.00
27749A Human ANGPTL4 Assay kit - IBL 96-Well $849.00
27750A Human ANGPTL3 (highly sensitive) Assay Kit - IBL 96-Well $606.00
 

 

 
 

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