The alpha-Klotho ELISA is a solid-phase sandwich ELISA that uses two highly specific antibodies to human alpha-Klotho protein. A mouse monoclonal Klotho antibody is precoated onto the Klotho ELISA plate; the other mouse monoclonal Klotho antibody is HRP-conjugated. The Klotho ELISA can be used to measure soluble human alpha-Klotho protein in human blood, serum and plasma.
The alpha-Klotho gene was originally identified in alpha-kl(-/-) genetically modified mice that show a phenotype closely associated with human aging, including symptoms of arteriosclerosis, osteoporosis, skin atrophy, pulmonary emphysema, depletion of Purkinje cells in the cerebellum and infertility after 3–4 weeks1. Because of this association, soluble alpha-Klotho protein is a useful parameter in aging studies and aging research.
Alpha-Klotho protein is a 130 kDa transmembrane protein involved in calcium homeostasis and vitamin D regulation. The protein is expressed primarily in the kidneys and parathyroid glands.
