- Simple method to identify insertions or deletions in mammalian cells
- Faster and more efficient than the surveyor assay; amplify directly from your target cells
- Complete kit: Guide-it Resolvase, Terra PCR Direct Polymerase and all buffers included
- Value: 100 reactions per kit
- Perfect for confirming sequence changes downstream from Cas9/CRISPR, TALENs or zinc-finger nuclease studies.
- Confirmation of mutations caused by Cas9/CRISPR, TALENs, or zinc-finger nucleases
- Detecting insertions and deletions in mammalian genomic DNA
- 4 x 5 ml Extraction Buffer 1
- 2 ml Extraction Buffer 2
- 100 µl Guide-it Resolvase
- 100 µl Terra PCR Direct Polymerase Mix (1.25 U/µl)
- 3 x 1 ml 2X Terra PCR Direct Buffer (with Mg2+, dNTP)
- 5 x 1 ml PCR-Grade Water
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.