The EpiScope Nucleosome Preparation Kit is designed for the preparation of nucleosomes from cultured mammalian cells. DNA is extracted from the prepared nucleosomes and analyzed using real-time PCR or sequencing to map nucleosome positions.
Nucleosomes are the constituent units of chromatin in eukaryotic nuclei, consisting of genomic DNA wrapped around a core histone complex1. Nucleosomes are not spaced at regular intervals on genomic DNA; instead, their positions are dependent on chromatin structure and transcriptional regulation2. In DNA methylation analysis, an important area in epigenetics research, nucleosome positioning has become a subject of intense interest. In transcriptionally-active genes, the promoter region is free of nucleosomes, permitting the binding of transcriptional initiation factors (TIFs). Conversely, DNA methylation within gene promoter regions leads to the formation of nucleosomes in those regions, preventing the binding of TIFs and suppressing transcriptional initiation.