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Products >  Cell_Biology_and_Epigenetics >  Epigenetics >  DNA_Methylation >  EpiScope_MSP

Methylation-Specific PCR: EpiScope MSP Kit

The EpiScope MSP Kit features a reagent designed exclusively for DNA methylation analysis via methylation-specific PCR (MSP) (Herman et al. 1996). This reagent contains a specific enzyme in an optimized buffer, allowing MSP analyses on bisulfite-treated uracil-containing DNA templates. This kit features high amplification efficiency and methylation specificity compared with standard PCR. The EpiScope MSP Kit is optimized for real-time monitoring using TB Green, enabling the performance of both real-time and endpoint PCR under the same reaction conditions.

Principle of MSP

Methlylation specific PCR identifies nucleotide regions in which cytosines have been altered to uracils following bisulfite treatment of the DNA template. These alterations are a function of the DNA template's CpG methylation status; methylated cytosines remain unaltered while the unmethylated cytosines are altered to uracils. Two primers are designed to detect these unaltered or altered regions, the methylated CpG DNA (M primer) and the unmethylated CpG DNA (UM primer), respectively. The last step involves methylation specific PCR.
  At-A-Glance   Documents   Images & Data   Resources

Features

  • High performance: Superior amplification speed and efficiency.
  • Specific: Differentiate methylated/unmethylated DNA by analyzing a single CpG site.
  • Streamlined: may be used for both real-time (TB Green) and endpoint PCR using the same PCR conditions.

Applications

  • Methylation-specific PCR for DNA methylation analysis

Components

2X MSP Buffer (with Mg2+ and dNTPs)*5 x 1 ml
MSP Enzyme240 µl
100X TB Green100 µl
ROX Reference Dye (50X conc.)200 µl
ROX Reference Dye II (50X conc.)200 µl

*The Mg2+ concentration (2X) is 4 mM and the dNTP concentration (2X) is 400 µM.

Storage

Store at –20°C

Note: The 100X TB Green dye is sensitive to light.

References

Herman, J. G., Graff, J. R., Myohanen, S., Nelkin, B. D. & Baylin, S. B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. 93, 9821-9826 (1996).

 
 
Products
Cat. # Product Contents Size Price License Units Select
R100A EpiScope® MSP Kit 200 Rxns $463.00 License Statements
R100B EpiScope® MSP Kit 400 Rxns $816.00 License Statements
 

 

 
 

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