c-MPL (TPOR) is a receptor for TPO and is a critical regulator of platelet formation and megakaryocytopoiesis. c-MPL/TPOR is a 635-amino acid transmembrane protein with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. Binding of the class I hematopoietic cytokine TPO causes c-MPL/TPOR to dimerize and activate the JAK/STAT pathway, thereby controlling a cell signaling pathway leading to production of platelets. c-MPL/TPOR may also play a role in hematopoietic stem cell maintenance in bone marrow.
Several human diseases are associated with mutations in the genes encoding thrombopoietin and c-MPL/TPOR. Mutations in THPO are known to result in hereditary thrombocythemia 1 (resulting in high blood platelet count) or some forms of leukemia. In mice, deficiency in c-MPL/TPOR results in severe thrombocytopenia, underscoring the role of c-MPL/TPOR in regulating megakaryocytopoiesis and platelet formation.
ELISAs for Thrombopoietin-Related Protein Detection
These c-MPL/TPOR ELISA or TPO ELISA kits enable detection of either mouse c-Myeloproliferative Leukemia protein (c-MPL), also known as Thrombopoietin Receptor (TPOR), mouse Cluster of Differentiation 110 (CD110), or human thrombopoietin (also known as TPO, THPO, or MGDF).
The Mouse c-MPL/TPOR Assay Kit allows sensitive, specific measurements of mouse soluble c-MPL/TPOR in serum or cell culture supernatant samples. The Human TPO Assay Kit* is a human TPO ELISA kit recommended for use with cell culture supernatant samples. Each ELISA kit provides materials needed to perform a solid phase sandwich ELISA using two highly specific antibodies, one precoated on the ELISA multi-well plate as a capture antibody and one HRP-conjugated as a detection antibody. Tetra Methyl Benzidine (TMB) is used as a substrate for detection.
*Cat. #27175A is not available in China, Taiwan, or Korea.
Antibodies for Thrombopoietin-Related Protein Detection
The Anti-Human TPO antibody is an affinity-purified IgG antibody that recognizes human thrombopoietin (TPO) protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) or immunohistochemical (IHC) detection of human TPO protein.