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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cytokines >  TNF-Alpha

TNF-Alpha Detection

Tumor necrosis factor (TNF or TNF-alpha) is a proinflammatory cytokine secreted primarily by macrophages. It is a member of the tumor necrosis factor ligand superfamily that regulates the acute-phase response to injury. In humans, TNF is a membrane-integrated protein that normally exists as homotrimers. TNF binds two receptors, TNF-R1 and TNF-R2, and activates three major signaling pathways: the NF-kappaB pathway, the MAP kinase pathway, and the death-signaling (apoptosis) cascade. TNF acts as a pyrogen that causes fever directly or by stimulating interleukin-1 (IL-1) secretion. It has been implicated in autoimmune diseases, insulin resistance, and cancer.

Alternate names for TNF include TNFA, tumor necrosis factor ligand superfamily member 2 (TNFSF2), DIF, APC1 protein, and cachectin.

ELISAs for TNF-Alpha Detection

The Rat TNF-alpha Assay Kit (Cat. # 27194) is a solid-phase sandwich ELISA using two antibodies that are highly specific to recombinant or native rat TNF protein; one antibody is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble rat TNF protein in cell culture supernatants.

Antibodies for TNF-Alpha Detection

The TNF-alpha antibody (Cat. # 18285A, B) is an affinity-purified IgG antibody that recognizes rat TNF protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of rat TNF protein.

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Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
TNF‑alpha
(C‑terminus)
27194AELISARatCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross-reactivity with rat IL-6, MCP-1, Osteopontin, and CINC-1
TNF‑alpha
(C‑terminus)
18285A, BIHC
WB
Rat(n/a)
Rabbit IgG
n/a

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
18285A Anti-Rat TNF-alpha Rabbit IgG Affinity Purify 10 ug $121.00
18285B Anti-Rat TNF-alpha Rabbit IgG Affinity Purify 100 ug $529.00
27194A Rat TNF- alpha Assay Kit - IBL 96-Well $644.00
 

 

 
 

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