Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cytokines >  NF_Kappa_B

NF-kappaB Detection

Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) is an ubiquitous transcription factor that was identified based on its interaction with an 11-base pair sequence in the immunoglobulin light-chain enhancer in B cells. NF-kappaB is involved in a variety of signaling processes, including responses to stress, cytokines, free radicals, ultraviolet radiation, and bacterial and viral antigens.

NF-kappaB consists of two subunits, each of which can exist as multiple isoforms. In humans, a 105-kDa precursor is encoded by the NFKB1 gene and is processed into a 50-kDa protein known as NF-kappaB p50. This subunit can form homodimers or heterodimers with other subunits; the most abundant NF-kappaB form consists of a p50-p65 heterodimer. In humans, a 65-kDa protein encoded by the RELA gene contributes to the most abundant form of NF-kappaB: a heterodimer of RELA/p65 and p50. NF-kappaB p65 interacts with many other transcription factors and contains a transactivation domain in its C-terminus. The inhibitor of NF-kappaB activity, I-kappaB, exerts its effect mostly through interaction with p65.

Alternate names for p50 include NFKB1, NFKB-p50, KBF1, EBP-1, DNA-binding factor KBF1, and nuclear factor NF-kappa-B p50 subunit.

Alternate names for p65 include v-rel reticuloendotheliosis viral oncogene homolog A (avian), NFKB3, NFKB-p65, NF-kappa-B p65delta3, transcription factor p65, and nuclear factor NF-kappa-B p65 subunit.

Antibodies for NF-kappaB Detection

The NF-kappaB p50 and p65 antibodies are affinity-purified IgG antibodies that recognize human NF-kappaB p50 and p65 proteins, respectively. The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of NF-kappaB p50 or p65 protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
NF‑kappaB p50
(N‑terminus)
18661A, BIHC
WB
Human(n/a)
Rabbit IgG
n/a
NF‑kappaB p50
(C‑terminus)
18663A, BIHC
WB
Human(n/a)
Rabbit IgG
n/a
NF‑kappaB p65
(N‑terminus)
18665A, BIHC
WB
Human(n/a)
Rabbit IgG
n/a
NF‑kappaB p65
(C‑terminus)
18667A, BIHC
WB
Human(n/a)
Rabbit IgG
n/a

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
18661A Anti-Human NFkappaB p50 (N) Rabbit IgG Affinity Purify 10 ug $113.00
18661B Anti-Human NFkappaB p50 (N) Rabbit IgG Affinity Purify 100 ug $499.00
18663A Anti-Human NFkappaB p50 (C) Rabbit IgG Affinity Purify 10 ug $113.00
18663B Anti-Human NFkappaB p50 (C) Rabbit IgG Affinity Purify 100 ug $499.00
18665A Anti-Human NFkappaB p65 (N) Rabbit IgG Affinity Purify 10 ug $113.00
18665B Anti-Human NFkappaB p65 (N) Rabbit IgG Affinity Purify 100 ug $499.00
18667A Anti-Human NFkappaB p65 (C) Rabbit IgG Affinity Purify 10 ug $113.00
18667B Anti-Human NFkappaB p65 (C) Rabbit IgG Affinity Purify 100 ug $499.00
 

 

 
 

Clontech is a Takara Bio Company © 2015 Privacy Policy | Terms & Conditions