Mouse keratinocyte-derived cytokine (KC) is the homolog of the human chemokine (C-X-C motif) ligand 1 (CXCL1) protein, a small cytokine belonging to the CXC chemokine subfamily. The synthesis of KC in vascular endothelial cells is induced by thrombin, and KC is involved in chemotaxis and activation of neutrophils and monocytes/macrophages. KC was originally identified by overexpression in murine keratinocytes, monocytes, and macrophages following stimulation by platelet-derived growth factor (PDGF) and macrophage colony-stimulating factor (M-CSF). Expression of KC is induced by mitogens in vascular smooth cells, endothelial cells, and macrophages. In mouse, KC activity is mediated by the interleukin-8 (IL-8) receptor (homologous to the human interleukin 8 (IL-8) type B receptor), which also binds mouse macrophage inflammatory protein-2 (MIP-2) with high affinity. These two chemokines have been suggested to be mouse homologs of human IL-8. Studies have shown that KC expression decreases the severity of multiple sclerosis, suggesting that KC may have a neuroprotective function.
Keratinocyte-derived cytokine was previously called growth-regulated alpha protein or GRO-alpha; alternative names include CXCL1, C-X-C motif chemokine 1, SCYB1, Fsp, and melanoma growth stimulating activity (MSGA).
ELISAs for Keratinocyte-Derived Cytokine Detection
The Mouse GRO/KC Assay Kit (Cat. # 27137A) is a solid-phase sandwich ELISA using two antibodies that are highly specific to mouse KC protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble mouse KC protein in serum, EDTA plasma, or cell culture supernatants.
Antibodies for Keratinocyte-Derived Cytokine Detection
KC (CXCL1) antibody products (Cat. # 18367A, B; 18361A, B; and 18366A) are affinity-purified IgG antibodies that recognize mouse keratinocyte chemoattractant (KC) or C-X-C motif chemokine 1 (CXCL1) protein. The anti-mouse KC antibodies were raised in rabbit using recombinant KC protein or synthetic peptides and are applicable to Western blot (WB) or immunohistochemical (IHC) detection of mouse KC protein.