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Products >  Cell_Biology_and_Epigenetics >  Cytokines >  Keratinocyte-Derived_Cytokine

Keratinocyte-Derived Cytokine Detection

Mouse keratinocyte-derived cytokine (KC) is the homolog of the human chemokine (C-X-C motif) ligand 1 (CXCL1) protein, a small cytokine belonging to the CXC chemokine subfamily. The synthesis of KC in vascular endothelial cells is induced by thrombin, and KC is involved in chemotaxis and activation of neutrophils and monocytes/macrophages. KC was originally identified by overexpression in murine keratinocytes, monocytes, and macrophages following stimulation by platelet-derived growth factor (PDGF) and macrophage colony-stimulating factor (M-CSF). Expression of KC is induced by mitogens in vascular smooth cells, endothelial cells, and macrophages. In mouse, KC activity is mediated by the interleukin-8 (IL-8) receptor (homologous to the human interleukin 8 (IL-8) type B receptor), which also binds mouse macrophage inflammatory protein-2 (MIP-2) with high affinity. These two chemokines have been suggested to be mouse homologs of human IL-8. Studies have shown that KC expression decreases the severity of multiple sclerosis, suggesting that KC may have a neuroprotective function.

Keratinocyte-derived cytokine was previously called growth-regulated alpha protein or GRO-alpha; alternative names include CXCL1, C-X-C motif chemokine 1, SCYB1, Fsp, and melanoma growth stimulating activity (MSGA).

ELISAs for Keratinocyte-Derived Cytokine Detection

The Mouse GRO/KC Assay Kit (Cat. # 27137A) is a solid-phase sandwich ELISA using two antibodies that are highly specific to mouse KC protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble mouse KC protein in serum, EDTA plasma, or cell culture supernatants.

Antibodies for Keratinocyte-Derived Cytokine Detection

KC (CXCL1) antibody products (Cat. # 18367A, B; 18361A, B; and 18366A) are affinity-purified IgG antibodies that recognize mouse keratinocyte chemoattractant (KC) or C-X-C motif chemokine 1 (CXCL1) protein. The anti-mouse KC antibodies were raised in rabbit using recombinant KC protein or synthetic peptides and are applicable to Western blot (WB) or immunohistochemical (IHC) detection of mouse KC protein.

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Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
KC27137AELISAMouseCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
0.5% cross‑reactivity with rat CINC-2-beta and ≤0.1% with mouse MIP-2, rat CINC-2-alpha, and rat CINC-3
KC18361A, BIHC
WB
Mouse(n/a)
Rabbit IgG
Cross-reacts with rat CINC-1; shows <0.1% cross-reactivity with human IL-8 and CXCL1; does not cross-react with rat CINC-2-alpha, CINC-2-beta, CINC-3, or mouse MIP-2
KC
(C‑terminus)
18366AIHC
WB
Mouse(n/a)
Rabbit IgG
Cross-reacts with rat CINC-1; shows <0.1% cross-reactivity with human IL-8 and CXCL1; does not cross-react with rat CINC-2-alpha, CINC-2-beta, CINC-3, or mouse MIP-2
KC
(N‑terminus)
18367A, BIHC
WB
Mouse(n/a)
Rabbit IgG
Cross-reacts with rat CINC-1; shows <0.1% cross-reactivity with human IL-8 and CXCL1; does not cross-react with rat CINC-2-alpha, CINC-2-beta, CINC-3, or mouse MIP-2

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
18367A Anti-Mouse KC (N) Rabbit IgG Affinity Purify 10 ug $117.00
18367B Anti-Mouse KC (N) Rabbit IgG Affinity Purify 100 ug $514.00
18361A Anti-Mouse KC Rabbit IgG 25 ug $117.00
18361B Anti-Mouse KC Rabbit IgG 500 ug $514.00
18366A Anti-Mouse KC (C) Rabbit IgG Affinity Purify 100 ug $514.00
27137A Mouse GRO/KC Assay Kit - IBL 96-Well $625.00
 

 

 
 

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