Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cytokines >  Interleukins

Interleukin Detection

Interleukins are a group of cytokines that were originally identified in white blood cells (leukocytes). It is now known that interleukins are expressed in a wide variety of cells and are involved in multiple cell-signaling pathways.

Interleukin 1 (IL-1) alpha and beta participate in the regulation of immune responses, inflammatory reactions, and hematopoiesis. IL-1 beta also plays a role in memory functions and regulation of the central nervous system (CNS), as well as cell proliferation, differentiation, and apoptosis. IL-1 beta is produced primarily in activated macrophages as an inactive precursor that is cleaved by caspase 1 to yield the active cytokine. It acts through two main receptors that also bind IL-1 alpha. Alternate names for IL-1 beta include catabolin, IL1B, and IL1F2.

Interleukin 6 (IL-6) is a cytokine that mediates a range of biological functions, including the inflammatory response and the maturation of B cells. IL-6 acts on B cells, T cells, hepatocytes, hematopoietic progenitor cells, and cells of the CNS. It is expressed at sites of acute and chronic inflammation, where it is secreted into the serum and binds the IL-6 receptor alpha. The IL-6 gene in humans is linked to many inflammation-associated disease states, including diabetes mellitus and juvenile rheumatoid arthritis. Studies have demonstrated that in humans IL-6 also acts as an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. Alternate names for IL-6 include interferon beta-2 (IFNB2), B-cell stimulatory factor-2 (BSF-2), CTL differentiation factor (CDF), B-cell stimulatory factor 2 (BSF2), B-cell differentiation factor, and hybridoma growth factor (HGF).

Interleukin 8 (IL-8) is a member of the CXC chemokine family. It is produced by macrophages and other cell types, such as epithelial cells, in response to an inflammatory stimulus. IL-8 functions primarily as a chemotactic factor to attract neutrophils, basophils, and T-cells, but not monocytes. It is also a potent angiogenic factor. Several cell surface receptors bind IL-8; the most studied are the G protein-coupled receptors (GPCRs), CXCR1 and CXCR2. IL-8 is associated with localized inflammation in gingivitis and psoriasis. Alternate names for IL-8 include chemokine (C-X-C motif) ligand 8, CXCL8, GCP-1, MDNCF, MONAP, NAP-1, GCP1, LECT, LUCT, LYNAP, NAF, NAP1, granulocyte chemotactic protein 1, monocyte-derived neutrophil chemotactic factor, monocyte-derived neutrophil-activating peptide, T-cell chemotactic factor, AMCF-I, K60, SCYB8, TSG-1, b-ENAP, alveolar macrophage chemotactic factor I, beta endothelial cell-derived neutrophil activating peptide, beta-thromboglobulin-like protein, lung giant cell carcinoma-derived chemotactic protein, lymphocyte derived neutrophil activating peptide, neutrophil-activating peptide 1, tumor necrosis factor-induced gene 1, emoctakin, C-X-C motif chemokine 8, neutrophil-activating protein 1, protein 3-10C, and small inducible cytokine subfamily B, member 8.

The Interleukin 2 receptor (also known as IL-2R) is a type I membrane protein that binds IL-2 and associates with JAK1. IL-2R is involved in signaling that affects T cell proliferation and is comprised of three subunits: alpha (CD25), beta (CD122), and gamma (CD132). Although the CD25 and CD122 subunits can each bind IL-2 as monomers, the heterodimer complex formed through association of these subunits has a faster association rate and a slower dissociation rate for IL-2 versus either subunit by itself. Once the CD25-CD122 dimer forms and binds IL-2, the CD132 subunit is recruited. Then, activation of the JAK/STAT pathway, the MAP kinase pathway, and the PI3K pathway can occur. IL-2R beta is also known as CD122, IL15RB, P75, and P70-75.

CD25 is expressed in activated T cells and B cells. CD25 expression is used as a marker to identify regulatory T cells (Tregs or CD4+CD25+ cells), cells that modulate the immune response and prevent autoimmunity. Increased CD25 expression in serum has been observed in patients with adult T cell leukemia and patients with malignant lymphoma.

ELISAs for Interleukin Detection

These products are solid-phase sandwich ELISAs using two antibodies that are highly specific to rat IL-1 beta or mouse IL-6 protein. One antibody is precoated on the ELISA plate and the other is HRP-conjugated. These kits can be used to measure soluble rat IL-1 beta or mouse IL-6 protein in serum, EDTA plasma, or cell culture supernatants.

Antibodies for CINC GRO Detection

The anti-rat IL-1 beta, rat IL-6, and human IL-8 antibodies are affinity-purified IgG antibodies that recognize rat IL-1 beta, rat IL-6, and human IL-8 proteins, respectively. The antibodies were raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC).

The anti-mouse CD122 monoclonal antibody (Cat. # 10399F) is an IL-2 receptor antibody that binds to the IL-2 receptor beta subunit and may be used for in vitro neutralization assays (NT) as well as inhibiting the association between IL-2 and IL-2R beta subunit in vivo.

The anti-human CD25 antibody products (Cat. # 10905A; # 10907A, B) are monoclonal antibodies raised in mouse and can be used to detect human CD25 cells by Western blot (WB), immunoprecipitation (IP), and flow cytometry (FCM).

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
IL-1 beta27193AELISARatCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross-reactivity with rat IL-1 alpha
IL-627768AELISAMouseCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross-reactivity with mouse leptin, MIP-2, and osteopontin
IL-1 beta
(C‑terminus)
18141A, BIHC
WB
Rat(n/a)
Rabbit IgG
n/a
IL-6
(N‑terminus)
18611A, BIHC
WB
Rat(n/a)
Rabbit IgG
n/a
IL-8
(C‑terminus)
18401A, BWBHuman(n/a)
Rabbit IgG
Does not cross react with human GRO, GRO alpha/MGSA, rat GRO/CINC-1, -2 alpha, or -2 beta
CD122/IL-2R beta10399FNTMouse(TM-beta 1)
Rat IgG MoAb
(Frozen in PBS; no additives)
n/a
CD25/IL-2R alpha10905AWB
IP
FCM
ELISA
Blocking
Human(H-8)
Mouse IgG MoAb
n/a
CD25/IL-2R alpha10907A, BWB
IP
FCM
ELISA
Human(H-48)
Mouse IgG MoAb
n/a

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
18141A Anti-Rat IL-1 beta (C) Rabbit IgG Affinity Purify 10 ug $113.00
18141B Anti-Rat IL-1 beta (C) Rabbit IgG Affinity Purify 100 ug $499.00
18401A Anti-Human IL-8 (C) Rabbit IgG Affinity Purify 10 ug $113.00
18401B Anti-Human IL-8 (C) Rabbit IgG Affinity Purify 100 ug $499.00
18611A Anti-Rat IL-6 (N) Rabbit IgG Affinity Purify 10 ug $113.00
18611B Anti-Rat IL-6 (N) Rabbit IgG Affinity Purify 100 ug $499.00
27768A Mouse IL-6 Assay Kit - IBL 96-Well $606.00
27193A Rat IL-1 beta Assay Kit - IBL 96-Well $606.00
10399F Anti-Mouse CD122/IL-2Rbeta (TM-beta1) Rat IgG MoAb (Frozen) 0.5 mg $1,000.00
10905A Anti-CD25 (H-8) Mouse IgG MoAb 100 ug $595.00
10907A Anti-CD25 (H-48) Mouse IgG MoAb 100 ug $595.00
10907B Anti-CD25 (H-48) Mouse IgG MoAb 500 ug $985.00
 

 

 
 

Clontech is a Takara Bio Company © 2015 Privacy Policy | Terms & Conditions