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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cytokines >  IFN_Gamma

IFN gamma ELISA Kits

Interferon gamma (IFN gamma, IFN-γ) is a cytokine produced by T cells, NK cells, and CD4+/CD8+ lymphocytes when they are activated by antigens, mitogens, or allogeneic antigens. IFN gamma plays an important role in immune and inflammatory responses by contributing to activation, growth, and division of T lymphocytes and various other cells and by strengthening MHC expression of antigen-presenting cells. IFN gamma also inhibits the IL-4-induced proliferation of B cells and inhibits the growth of smooth muscle cells of the arterial intima in vitro and in vivo. Finally, IFN gamma is hypothesized to inhibit excessive vascular expansion in response to arterial damage caused by stenosis.

These sandwich-type EIA kits can be used to quantitatively detect pig (Cat. # MK154), human (Cat. # MK153), or mouse (Cat. # MK152) IFN gamma in serum, plasma, or cell culture supernatant.

  At-A-Glance   Documents

Features

  • ELISA kits for the quantitative detection of pig, human, or mouse IFN gamma.

Applications

  • Measurement of pig (Cat. # MK154), human (Cat. # MK153), or mouse (Cat. # MK152) IFN gamma in serum and plasma samples, and in cell culture supernatant.

Components

Cat. # MK152

Antibody Coated Microtiterplate96 well x 1
Wash Buffer Concentrate (X20)25 ml
Standard mouse IFN (8 ng)For 1 ml x 2
Assay Diluent A30 ml
Assay Diluent B (X5)15 ml
Detection Antibody mouse IFNFor 0.1 ml x 2
HRP-Streptavidin Concentrate0.2 ml
Substrate Solution (TMBZ)12 ml
Stop Solution without Sulfuric Acid12 ml

Cat. # MK153

Antibody Coated Microtiterplate96 well x 1
Wash Buffer Concentrate (X20)25 ml
Standard human IFN (20 ng)For 1 ml x 2
Assay Diluent A30 ml
Assay Diluent B (X5)15 ml
Detection Antibody human IFNFor 0.1 ml x 2
HRP-Streptavidin Concentrate0.2 ml
Substrate Solution (TMBZ)12 ml
Stop Solution without Sulfuric Acid12 ml

Cat. # MK154

Antibody Coated Microtiterplate96 well x 1
Wash Buffer Concentrate (X20)25 ml
Standard pig IFN (120 ng)For 0.5 ml x 2
Assay Diluent C30 ml
Assay Diluent B (X5)15 ml
Detection Antibody pig IFNFor 0.1 ml x 2
HRP-Streptavidin Concentrate0.2 ml
Substrate Solution (TMBZ)12 ml
Stop Solution without Sulfuric Acid12 ml

Storage

–20°C

Performance

  • Sensitivity: pig (Cat. # MK154): 3.75 ng/ml; human (Cat. # MK153): 78 pg/ml; mouse (Cat. # MK152): 63 pg/ml
  • Assay duration: pig (Cat. # MK154): ~4 hours; human (Cat. # MK153): ~3 hours; mouse (Cat. # MK152): ~3 hours
  • Test specimen type: Serum, plasma, and cell culture supernatants
  • Sample volume per well: 100 µl

 
 
Products
Cat. # Product Contents Size Price Units Select
MK152 Mouse IFN gamma EIA Kit 96-Well $787.00
MK153 Human IFN gamma EIA Kit 96-Well $787.00
MK154 Pig IFN gamma EIA Kit 96-Well $787.00
 

 

 
 

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