Granulocyte colony-stimulating factor (G-CSF), encoded by the CSF3 gene in humans, is a glycoprotein, growth factor, and cytokine that controls the production, differentiation, and function of granulocytes. G-CSF stimulates the production of granulocytes from stem cells in bone marrow and their subsequent release into the circulatory system. G-CSF is expressed in endothelial cells, macrophages, and several other types of immune cells. G-CSF also regulates the differentiation and propagation of neutrophil precursors using a variety of pathways mediated by kinases such as JAK, STAT, MAPK, PI3K, and protein kinase B. Recombinant G-CSF is used in therapy for some cancer patients to assist in recovery from neutropenia.
Alternate names for G-CSF include GCSF, CSF-3, filgrastim, lenograstim, and pluripoietin
ELISAs for G-CSF Detection
The Human G-CSF Assay Kit (Cat. # 27131A) is a solid-phase sandwich ELISA using two antibodies that are highly specific to human G-CSF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human G-CSF protein in cell culture supernatants.
Antibodies for G-CSF Detection
Anti-human G-CSF antibody products (Cat. # 10111A; # 11041A, B) are affinity-purified IgG antibodies that recognize human G-CSF protein. G-CSF antibodies were raised in mouse using recombinant human G-CSF protein and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or neutralization assay (NT) of human G-CSF protein.