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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cytokines >  G-CSF

G-CSF Detection

Granulocyte colony-stimulating factor (G-CSF), encoded by the CSF3 gene in humans, is a glycoprotein, growth factor, and cytokine that controls the production, differentiation, and function of granulocytes. G-CSF stimulates the production of granulocytes from stem cells in bone marrow and their subsequent release into the circulatory system. G-CSF is expressed in endothelial cells, macrophages, and several other types of immune cells. G-CSF also regulates the differentiation and propagation of neutrophil precursors using a variety of pathways mediated by kinases such as JAK, STAT, MAPK, PI3K, and protein kinase B. Recombinant G-CSF is used in therapy for some cancer patients to assist in recovery from neutropenia.

Alternate names for G-CSF include GCSF, CSF-3, filgrastim, lenograstim, and pluripoietin

ELISAs for G-CSF Detection

The Human G-CSF Assay Kit (Cat. # 27131A) is a solid-phase sandwich ELISA using two antibodies that are highly specific to human G-CSF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human G-CSF protein in cell culture supernatants.

Antibodies for G-CSF Detection

Anti-human G-CSF antibody products (Cat. # 10111A; # 11041A, B) are affinity-purified IgG antibodies that recognize human G-CSF protein. G-CSF antibodies were raised in mouse using recombinant human G-CSF protein and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or neutralization assay (NT) of human G-CSF protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
G-CSF27131AELISAHumanCapture:
(n/a)
Mouse IgG MoAb

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross‑reactivity with human GM-CSF
G-CSF10111AWB
ELISA
NT
Human(4-12-2)
Mouse IgG MoAb
n/a
G-CSF11041A, BIHCHuman(4-12-2)
Mouse IgG MoAb
n/a

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
27131A Human G-CSF Assay Kit - IBL 96-Well $606.00
10111A Anti-Human G-CSF (4-12-2) Mouse IgG MoAb 500 ug $499.00
11041A Anti-Human G-CSF (4-12-2) Mouse IgG MoAb 1 mL $499.00
11041B Anti-Human G-CSF (4-12-2) Mouse IgG MoAb 0.1 mL $114.00
 

 

 
 

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