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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cytokines >  CXCL1_CXCL2

CXCL1 and CXCL2 Detection

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine subfamily that is expressed by macrophages, neutrophils, and epithelial cells. Also known as GRO-alpha, it is secreted by human melanoma cells, has mitogenic properties, and is implicated in melanoma pathogenesis. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors. It also acts as a chemoattractant for neutrophils and is involved in angiogenesis, inflammation, wound healing, and tumorigenesis. CXCL1 acts by signaling through the chemokine receptor CXCR2. Studies in mice have shown that CXCL1 decreased the severity of multiple sclerosis and may have a neuroprotective function. CXCL1 was previously known as GRO1 oncogene, GRO-alpha, fibroblast secretory protein (FSP), and melanoma growth stimulating activity alpha (MSGA-alpha); currently, alternate names include neutrophil-activating protein 3 (NAP-3), growth-regulated alpha protein, C-X-C motif chemokine 1, SCYB1, and GROA.

CXCL2 (also known as GRO-beta) has 90% sequence identity with CXCL1. CXCL2 is expressed by activated monocytes and neutrophils at sites of inflammation. It exhibits chemotactic activity with polymorphonuclear leukocytes and hematopoietic stem cells. CXCL2 is known to interact with a cell-surface chemokine receptor, CXCR2.

Mouse macrophage inflammatory protein 2 (MIP-2) is the homolog of the human CXCL2 protein and the rat CINC-2 protein. MIP-2 is expressed by activated monocytes and neutrophils at sites of inflammation. It has also been shown to control mucosal lymphocyte migration in mice. Alternate names for CXCL2 include MIP-2-alpha; previously it was known as GRO2 oncogene, GRO-beta, and melanoma growth stimulating activity beta (MSGA-beta).

ELISAs for CXCL1 and CXCL2 Detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human CXCL1 (GRO-alpha) protein, human CXCL2 protein, or mouse MIP-2 protein; one antibody is precoated on the ELISA plate and the other is HRP-conjugated. These assays can be used to measure soluble protein in serum, EDTA plasma, or cell culture supernatants.

Antibodies for CXCL1 and CXCL2 Detection

These products are affinity-purified IgG antibodies that recognizes human CXCL1 or CXCL2 protein. The antibodies were raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human CXCL1 or CXCL2 protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
CXCL127139AELISAHumanCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross‑reactivity with human IL-8, IP-10, and rat GRO-alpha (CINC-1)
CXCL2 (C‑terminus)27142AELISAHumanCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
1.7% cross‑reactivity with human CXCL1
MIP-227138AELISAMouseCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross-reactivity with rat CINC-2-alpha, CINC-2-beta, and mouse KC
CXCL1 (C‑terminus)18356A, BIHC
WB
Human(n/a)
Rabbit IgG
Does not cross-react with human GRO-beta (CINC-3), IL-8, IP-10; does not cross-react with rat GRO/CINC-1, CINC-2-alpha, CINC-2-beta, or CINC-3
CXCL2 (C‑terminus)18365A, BIHC
WB
Human(n/a)
Rabbit IgG
Does not cross-react with human CXCL1

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
18356A Anti-Human GRO alpha /MGSA (C) Rabbit IgG Affinity Purify 10 ug $113.00
18356B Anti-Human GRO alpha /MGSA (C) Rabbit IgG Affinity Purify 100 ug $499.00
18365A Anti-Human GRO beta (C) Rabbit IgG Affinity Purify 10 ug $113.00
18365B Anti-Human GRO beta (C) Rabbit IgG Affinity Purify 100 ug $499.00
27138A Mouse GRO beta/MIP-2 Assay Kit - IBL 96-Well $606.00
27139A Human GRO alpha/MGSA Assay Kit - IBL 96-Well $606.00
27142A Human GRO beta Assay Kit - IBL 96-Well $606.00
 

 

 
 

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