Rat cytokine-induced neutrophil chemoattractant-1 (CINC-1) protein is a member of the CXC family of chemokines. The rat CINC family members, including CINC-1, CINC-2-alpha, CINC-2-beta, and CINC-3, are homologous to human GRO/CXCL and mouse MIP-2/KC genes. CINC-1 possesses high sequence similarity to IL-8, and similarly induce leukocyte chemotaxis. CINC-1 also has high similarity to mouse keratinocyte-derived cytokine (KC). Rat CINC-1 is also known as chemokine (C-X-C motif) ligand 1 (CXCL1), GRO-alpha, and melanoma growth stimulating activity alpha (MGSA alpha).
In addition to its established role as a neutrophil chemoattractant, CINC-1 functions as an early acute-phase protein. Levels of CINC-1 in the liver and serum rise in response to brain injury, and CINC-1 is suggested to be responsible for recruitment of neutrophils to the site of injury.
Rat CINC-2 and CINC-3 are also members of the CXC family of chemokines. Rat CINC-2-alpha and CINC-2-beta, differing by only three C-terminal residues, were originally identified as novel neutrophil chemoattractants in conditioned medium of granulation tissue obtained from carrageenan-induced inflammation. Mature CINC-2-beta is a 68-amino acid protein generated by cleavage of a 32-residue signal peptide. CINC-2-alpha and CINC-2-beta have similar chemotactic activity, and they contribute to neutrophil infiltration into inflammatory sites in rats. Other cell types that express CINC-2 proteins include activated macrophages and fibroblasts. Rat CINC-3 is expressed in monocytes and macrophages, endothelial cells, and fibroblasts. CINC-3 interacts with a cell-surface G-protein-coupled receptor known as CXCR2.
ELISAs for CINC GRO Detection
The Rat GRO/CINC-1 Assay Kit - IBL (Cat. # 27162A) is a solid-phase sandwich ELISA using two antibodies that are highly specific to rat CINC-1 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble rat CINC-1 protein in serum, EDTA plasma, or cell culture supernatants.
The rat GRO-CINC-2-alpha (Cat. # 17170A) and GRO/CINC-2-beta (Cat. # 17166A) assay kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to rat CINC-2 proteins; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble rat CINC-2-alpha or CINC-2-beta protein in serum or cell culture supernatants.
The rat GRO/CINC-3 (GRO beta/MIP-2) assay kit (Cat. # 27163A) is a solid-phase sandwich ELISA using two antibodies that are highly specific to rat CINC-3 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble rat CINC-3 protein in serum, EDTA-plasma, or cell culture supernatants.
Antibodies for CINC GRO Detection
The GRO/CINC-1 antibody products (Cat. # 10071A; 10072A, B; 18256B; 18257A, B; and 18251A, B) recognize rat CINC-1 protein. The antibodies were raised in either mouse (Cat. # 10071A and 10072A, B) or rabbit (Cat. # 18256B; 18257A, B; and 18251A, B) using synthetic peptides and can be used for Western blot (WB) detection, neutralization tests (NT), or immunohistochemical (IHC) detection of rat CINC-1 protein.
The GRO/CINC-2 antibody products (Cat. # 18275A, B; 18276A; and 18277A) are affinity-purified IgG antibodies that recognize rat CINC-2 proteins. The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection of rat CINC-2 proteins.
The GRO/CINC-3 antibody products (Cat. # 18291A and 18292A) are affinity-purified IgG antibodies that recognize rat CINC-3 protein. The antibodies were raised in rabbit using a recombinant protein or synthetic peptide and can be used for Western blot (WB) detection of rat CINC-3 protein.
