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Products >  Cell_Biology_and_Epigenetics >  Cytokines >  CINC-1_GRO

CINC GRO Detection

Rat cytokine-induced neutrophil chemoattractant-1 (CINC-1) protein is a member of the CXC family of chemokines. The rat CINC family members, including CINC-1, CINC-2-alpha, CINC-2-beta, and CINC-3, are homologous to human GRO/CXCL and mouse MIP-2/KC genes. CINC-1 possesses high sequence similarity to IL-8, and similarly induce leukocyte chemotaxis. CINC-1 also has high similarity to mouse keratinocyte-derived cytokine (KC). Rat CINC-1 is also known as chemokine (C-X-C motif) ligand 1 (CXCL1), GRO-alpha, and melanoma growth stimulating activity alpha (MGSA alpha).

In addition to its established role as a neutrophil chemoattractant, CINC-1 functions as an early acute-phase protein. Levels of CINC-1 in the liver and serum rise in response to brain injury, and CINC-1 is suggested to be responsible for recruitment of neutrophils to the site of injury.

Rat CINC-2 and CINC-3 are also members of the CXC family of chemokines. Rat CINC-2-alpha and CINC-2-beta, differing by only three C-terminal residues, were originally identified as novel neutrophil chemoattractants in conditioned medium of granulation tissue obtained from carrageenan-induced inflammation. Mature CINC-2-beta is a 68-amino acid protein generated by cleavage of a 32-residue signal peptide. CINC-2-alpha and CINC-2-beta have similar chemotactic activity, and they contribute to neutrophil infiltration into inflammatory sites in rats. Other cell types that express CINC-2 proteins include activated macrophages and fibroblasts. Rat CINC-3 is expressed in monocytes and macrophages, endothelial cells, and fibroblasts. CINC-3 interacts with a cell-surface G-protein-coupled receptor known as CXCR2.

ELISAs for CINC GRO Detection

The Rat GRO/CINC-1 Assay Kit - IBL (Cat. # 27162A) is a solid-phase sandwich ELISA using two antibodies that are highly specific to rat CINC-1 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble rat CINC-1 protein in serum, EDTA plasma, or cell culture supernatants.

The rat GRO-CINC-2-alpha (Cat. # 17170A) and GRO/CINC-2-beta (Cat. # 17166A) assay kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to rat CINC-2 proteins; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble rat CINC-2-alpha or CINC-2-beta protein in serum or cell culture supernatants.

The rat GRO/CINC-3 (GRO beta/MIP-2) assay kit (Cat. # 27163A) is a solid-phase sandwich ELISA using two antibodies that are highly specific to rat CINC-3 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble rat CINC-3 protein in serum, EDTA-plasma, or cell culture supernatants.

Antibodies for CINC GRO Detection

The GRO/CINC-1 antibody products (Cat. # 10071A; 10072A, B; 18256B; 18257A, B; and 18251A, B) recognize rat CINC-1 protein. The antibodies were raised in either mouse (Cat. # 10071A and 10072A, B) or rabbit (Cat. # 18256B; 18257A, B; and 18251A, B) using synthetic peptides and can be used for Western blot (WB) detection, neutralization tests (NT), or immunohistochemical (IHC) detection of rat CINC-1 protein.

The GRO/CINC-2 antibody products (Cat. # 18275A, B; 18276A; and 18277A) are affinity-purified IgG antibodies that recognize rat CINC-2 proteins. The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection of rat CINC-2 proteins.

The GRO/CINC-3 antibody products (Cat. # 18291A and 18292A) are affinity-purified IgG antibodies that recognize rat CINC-3 protein. The antibodies were raised in rabbit using a recombinant protein or synthetic peptide and can be used for Western blot (WB) detection of rat CINC-3 protein.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
CINC-127162AELISARatCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross-reactivity with rat CINC-2-alpha, CINC-2-beta, CINC-3, and mouse GRO-beta/MIP-2

Cross-reacts with mouse CINC-1
CINC-2-alpha17170AELISARatCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross-reactivity with other rat CINC isoforms, rat MCP-1, rat Rantes, rat MIP-1-alpha, and rat IL-1-beta
CINC-1
(C-terminus)
10071AWBRat(6G2)
Mouse IgM
Does not cross-react with N-terminal rat CINC-1, rat CINC-2-alpha, rat CINC-2-beta, rat CINC-3, mouse MIP-2, human GRO-alpha, or human IL-8
CINC-2-beta17166AELISARatCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross-reactivity with rat GRO/CINC-2-alpha, GRO/CINC-1 and -3, MCP-1, Rantes, MIP-1-alpha, and IL-1-beta
CINC-3-beta27163AELISARatCapture:
(n/a)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross-reactivity with rat GRO/CINC-1, -2-alpha, -2-beta, and mouse GRO/KC
CINC-3-beta
(N‑terminus)
10072A, BWB
NT
Rat(2A9)
Mouse IgG
Does not cross-react with C-terminal rat CINC-1, rat CINC-2-alpha, rat CINC-2-beta, rat CINC-3, mouse MIP-2, human GRO-alpha, or human IL-8
CINC-118251A, BIHC
WB
Rat(n/a)
Rabbit IgG
Cross-reacts with mouse KC; does not cross-react with rat CINC-2-alpha, rat CINC-2-beta, rat CINC-3, or mouse MIP-2
CINC-1
(C-terminus)
18256BWB
NT
Rat(n/a)
Rabbit IgG
Does not cross-react with rat CINC-2-alpha, rat CINC-2-beta, or rat CINC-3
CINC-1
(N-terminus)
18257A, BIHC
WB
NT
Rat(n/a)
Rabbit IgG
Does not cross-react with rat CINC-2-alpha, rat CINC-2-beta, or rat CINC-3
CINC-2-alpha
(C-terminus)
18275A, BWBRat(n/a)
Rabbit IgG
Does not cross-react with rat CINC-1, rat CINC-2-beta, or rat CINC-3
CINC-2-beta
(C-terminus)
18276AWBRat(n/a)
Rabbit IgG
Does not cross-react with rat CINC-1, rat CINC-2-alpha, or rat CINC-3
CINC-2-alpha and ‑beta (common
N-terminal region)
18277AWBRat(n/a)
Rabbit IgG
Does not cross-react with rat CINC-1 or rat CINC-3
CINC-318291AWBRat(n/a)
Rabbit IgG
Cross-reacts with mouse MIP-2; does not cross-react with rat CINC-1, rat CINC-2-alpha, or rat CINC-2-beta
CINC-3
(C-terminus)
18292AWBRat(n/a)
Rabbit IgG
Cross-reacts with mouse MIP-2; does not cross-react with rat CINC-1, rat CINC-2-alpha, rat CINC-2-beta, or mouse KC

 

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
10071A Anti-Rat GRO/CINC-1 (6G2) (C) Mouse IgM MoAb 500 ug $499.00
10072A Anti-Rat GRO/CINC-1 (2A9) (N) Mouse IgG MoAb 10 ug $113.00
10072B Anti-Rat GRO/CINC-1 (2A9) (N) Mouse IgG MoAb 500 ug $499.00
18256B Anti-Rat GRO/CINC-1 (C) Rabbit IgG Affinity Purify 100 ug $499.00
18257A Anti-Rat GRO/CINC-1 (N) Rabbit IgG Affinity Purify 10 ug $113.00
18275A Anti-Rat GRO/CINC-2 alpha(C) Rabbit IgG Affinity Purify 10 ug $113.00
18275B Anti-Rat GRO/CINC-2 alpha(C) Rabbit IgG Affinity Purify 100 ug $499.00
18276A Anti-Rat GRO/CINC-2 beta (C) Rabbit IgG Affinity Purify 100 ug $499.00
18277A Anti-Rat GRO/CINC-2 alpha, 2 beta (N) Rabbit IgG Affinity Purify 100 ug $499.00
18291A Anti-Rat GRO/CINC-3 Rabbit IgG 500 ug $499.00
18292A Anti-Rat GRO/CINC-3 (C) Rabbit IgG Affinity Purify 100 ug $499.00
17166A Rat GRO/CINC-2 beta Assay Kit - IBL 96-Well $606.00
17170A Rat GRO/CINC-2 alpha Assay Kit - IBL 96-Well $606.00
27162A Rat GRO/CINC-1 Assay Kit - IBL 96-Well $606.00
27163A Rat GRO/CINC-3 (GRO beta/MIP-2) Assay Kit - IBL 96-Well $606.00
18257B Anti-Rat GRO/CINC-1 (N) Rabbit IgG Affinity Purify 100 ug $499.00
18251A Anti-Rat GRO/CINC-1 Rabbit IgG 25 ug $113.00
18251B Anti-Rat GRO/CINC-1 Rabbit IgG 500 ug $499.00
 

 

 
 

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