Cell death can be assayed by quantifying plasma membrane damage or rupture. This LDH assay kit offers a simple way to measure plasma membrane damage, based on the release of lactate dehydrogenase (LDH), a stable cytoplasmic enzyme present in most cells.
Highly Sensitive & Accurate
The LDH Cytotoxicity Detection Kit is a simple and accurate colorimetric assay for dead and damaged cells. LDH present in the culture supernatant (due to plasma membrane damage) participates in a coupled reaction which converts a yellow tetrazolium salt into a red, formazan-class dye which is measured by absorbance at 492 nm. The amount of formazan is directly proportional to the amount of LDH in the culture, which is in turn directly proportional to the number of dead or damaged cells. The assay is extremely sensitive: as few as 2,000 dead or damaged cells per well can be detected. Results of LDH and [51Cr] assays for cell-mediated cytotoxicity correlate strongly with each other.
Simple Procedure, Fast Results
The LDH assay does not require prelabeling or washing steps. The entire procedure, from cell growth to measuring results, can be performed in a single 96-well plate. It takes less than an hour once your cells are cultured: Simply add the reaction mixture, incubate for 10–30 minutes, and measure the absorbance at 492 nm with a multiwell plate reader. Additionally, the assay is nonradioactive for improved safety and simplified cleanup and waste disposal.
In Vitro Applications
LDH assays have been used to quantify cell-mediated cytotoxicity induced by cytotoxic T cells, natural killer cells, lymphokine-activated killer cells, and monocytes, as well as to identify mediators that induce cytolysis. LDH assays have also been used to determine the cytotoxic potential of compounds in environmental and medical research and in food, cosmetic, and pharmaceutical manufacturing, and to detect cell death in bioreactors.