Laminin (LN) proteins are part of a large family of extracellular matrix basal laminal glycoproteins and play important roles in cell adhesion, growth, communication, and tumor metastasis. LN consists of alpha, beta, and gamma polypeptide chains that are linked by disulfide bridges to form a characteristic asymmetric cross-structure that has been observed via electron microscopy. LN also binds to various components of the basal membrane and is hypothesized to link these components together. Cell surface receptors that are thought to play a role in LN-mediated cell adhesion have been isolated from platelets and metastatic tumor cells. Furthermore, levels of LN fragments in serum has been found to be elevated in patients with hepatic fibrosis, alcoholic liver, hypertension, and several tumor types.
ELISAs for Laminin Detection
The Laminin EIA Kit is a solid-phase sandwich ELISA that utilizes two specific mouse monoclonal LN antibodies; one is coated on the plate and the other is peroxidase-labeled. This 96-well in vitro assay is intended for the quantification of human basal laminal glycoprotein in serum, plasma, urine, cultured cell extract, cell culture supernatants, and other biological fluids.
Antibodies for Laminin Detection
Human Laminin (LN) Antibody (Clone LN82-13) is designed to detect basal laminal glycoprotein via Western blot (WB) analysis under non-reducing and non-heating conditions, as well as via immunohistochemistry (IHC) of frozen and paraffin-embedded tissue sections.
