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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cell_Adhesion_and_Extracellular_Matrix >  Laminin

Laminin Detection

Laminin (LN) proteins are part of a large family of extracellular matrix basal laminal glycoproteins and play important roles in cell adhesion, growth, communication, and tumor metastasis. LN consists of alpha, beta, and gamma polypeptide chains that are linked by disulfide bridges to form a characteristic asymmetric cross-structure that has been observed via electron microscopy. LN also binds to various components of the basal membrane and is hypothesized to link these components together. Cell surface receptors that are thought to play a role in LN-mediated cell adhesion have been isolated from platelets and metastatic tumor cells. Furthermore, levels of LN fragments in serum has been found to be elevated in patients with hepatic fibrosis, alcoholic liver, hypertension, and several tumor types.

ELISAs for Laminin Detection

The Laminin EIA Kit is a solid-phase sandwich ELISA that utilizes two specific mouse monoclonal LN antibodies; one is coated on the plate and the other is peroxidase-labeled. This 96-well in vitro assay is intended for the quantification of human basal laminal glycoprotein in serum, plasma, urine, cultured cell extract, cell culture supernatants, and other biological fluids.

Antibodies for Laminin Detection

Human Laminin (LN) Antibody (Clone LN82-13) is designed to detect basal laminal glycoprotein via Western blot (WB) analysis under non-reducing and non-heating conditions, as well as via immunohistochemistry (IHC) of frozen and paraffin-embedded tissue sections.

  At-A-Glance   Documents   Images & Data   Resources

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
LamininMK107ELISAHumanCapture:
(n/a)
Mouse MoAb

Detect: HRP (n/a)
Mouse MoAb
No reactivity with human fibronectin, vitronectin, fibrinogen, or collagen types I and III
Laminin gamma 1 chain (N‑terminus)M020IHC
WB
Human(LN82-13)
Mouse IgG MoAb
Cross-reacts with rabbit laminin

Does not cross-react with mouse laminin

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
MK107 Laminin EIA Kit 96 Rxns $540.00
M020 Monoclonal Anti-Human Laminin (Clone LN82-13) 0.1 mg $357.00
 

 

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Cat. # Product Contents Size Price Units Select
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