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Cell Biology & Epigenetics


Products >  Cell_Biology_and_Epigenetics >  Cancer_and_Inflammation >  VEGF

VEGF Detection

The vascular endothelial growth factor (VEGF) family of cell signaling proteins is responsible for a range of processes in the development and maintenance of adult tissues. Members of the VEGF family include placental growth factor (PGF), VEGF-A, VEGF-B, VEGF-C, and VEGF-D. Products are available to detect VEGF-A, VEGF-C, and VEGFR-3/FLT4, the receptor that binds VEGF-C and VEG-F-D.

Human VEGF-A is a 38-kDa homodimeric protein that consists of two 165 amino acid polypeptide chains. VEGF-A promotes embryonic development of the circulatory system (vasculogenesis) and new blood vessel formation from existing vasculature (angiogenesis). It also regulates vascular permeability, endothelial cell growth, cell migration, and apoptosis. VEGF-A is expressed in many tumor samples; VEGF-mediated vascularization enables tumors to grow rapidly. Mutations in VEGF-A are associated with proliferative and nonproliferative diabetic retinopathy. Alternate names for VEGF-A include VEGF, vascular permeability factor (VPF), and MVCD1.

VEGF-C undergoes complex post-translational processing, generating multiple isoforms that bind to and activate VEGFR-2 and VEGFR-3 receptors. Only the fully processed form can bind the VEGFR-2 receptor. VEGF-C regulates vascular permeability as well as endothelial cell growth and migration. It may also control angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and it may maintain the differentiated lymphatic endothelium in adults. Alternate names for VEGF-C include vascular endothelial growth factor-related protein (VRP), FLT4 ligand DHM, and Flt4 ligand (Flt4-L).

Vascular endothelial growth factor receptor 3 (VEGFR-3), encoded by the human FLT4 gene, is a cell-surface tyrosine kinase receptor that binds VEGF-C and VEGF-D. It is involved in adult lymphangiogenesis and maintenance of the lymphatic endothelium. Activation of VEGFR-3/FLT4 results in upregulation of VEGF-C expression, creating a positive feedback loop. VEGFR-3/FLT4 activates several downstream signaling pathways through the action of MAPK1/ERK2, MAPK3/ERK1, MAPK8, c-Jun, and AKT1. Mutations in the FLT4 gene are associated with hereditary lymphedema type IA. Alternate names for VEGFR-3 include fms-related tyrosine kinase 4, PCL, LMPH1A, and FLT41.

ELISAs for VEGF Detection

The VEGF assay kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human VEGF, VEGF-C, or VEGFR-3/Flt-4 proteins, mouse VEGF protein, or rat VEGF protein; the capture antibody is precoated on the ELISA plate, and the detection antibody is HRP-conjugated. These kits can be used to measure soluble human, mouse, or rat VEGF proteins in EDTA plasma, heparin plasma, or cell culture supernatants.

Antibodies for VEGF Detection

VEGF antibody products are affinity-purified VEGF IgG antibodies that recognize human or rat VEGF-A or VEGF-C protein. These antibodies were raised in rabbit or mouse using a synthetic peptide or recombinant protein and can be used to detect VEGF-A or VEGF-C protein by Western blot (WB) or immunohistochemistry (IHC).

  At-A-Glance   Documents   Images & Data   Resources

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
Cross‑Reactivity
VEGF‑16527171AELISAHumanCapture:
(16F1)
Mouse IgG MoAb

Detect: HRP
(n/a)
Rabbit IgG
39.7% cross‑reactivity with human VEGF‑121

≤0.1% cross-reactivity with human PDGF
VEGF-16427101AELISARatCapture:
(V-N)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross‑reactivity with human PDGF
VEGF-16427102AELISAMouseCapture:
(V-N)
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG
≤0.1% cross‑reactivity with human VEGF-165
VEGF-C27756AELISAHumanCapture:
(13C1)
Mouse IgG MoAb

Detect: HRP
(408)
Rabbit IgG
n/a
VEGFR‑3/FLT427779AELISAHumanCapture:
(17A1)
Mouse IgG MoAb

Detect: HRP
(32A1)
Mouse IgG MoAb
≤0.1% cross-reactivity with human c-Kit, KDR, Tie-2, PDGFR-alpha, and PDGFR-beta
VEGF10053AWBHuman(16F1)
Mouse IgG MoAb
n/a
VEGF11081A, BIHC
WB
Human(2E1)
Mouse IgG MoAb
n/a
VEGF11084CIHCHuman(R11)
Mouse IgG MoAb
n/a
VEGF11085A, BIHCHuman(N5)
Mouse IgG MoAb
n/a
VEGF (N‑terminus)18411A, BIHC
WB
Human(V-N)
Rabbit IgG
n/a
VEGF (conjugated to bovine thyroglobulin)18413A, BIHC
WB
Human(V-3)
Rabbit IgG
n/a
VEGF-C (N‑terminus)18415A, BIHC
WB
Human(103)
Rabbit IgG
n/a
VEGF (N‑terminus)18420A, BWBRat(V-N)
Rabbit IgG
n/a

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
10053A Anti-Human VEGF (16F1) Mouse IgG MoAb 500 ug $499.00
11081A Anti-Human VEGF (2E1) Mouse IgG MoAb 50 ug $113.00
11081B Anti-Human VEGF (2E1) Mouse IgG MoAb 500 ug $499.00
11084C Anti-Human VEGF (R11) Mouse IgG MoAb 100 ug $499.00
11085A Anti-Human VEGF (N5) Mouse IgG MoAb 200 ug $499.00
11085B Anti-Human VEGF (N5) Mouse IgG MoAb 500 ug $1,000.00
18411A Anti-Human VEGF (V-N) Rabbit IgG Affinity Purify 10 ug $113.00
18411B Anti-Human VEGF (V-N) Rabbit IgG Affinity Purify 100 ug $499.00
18413A Anti-Human VEGF (V-3) Rabbit IgG Affinity Purify 10 ug $113.00
18413B Anti-Human VEGF (V-3) Rabbit IgG Affinity Purify 100 ug $499.00
27101A Rat VEGF Assay Kit - IBL 96-Well $606.00
27102A Mouse VEGF Assay Kit - IBL 96-Well $606.00
27756A Human VEGF-C Assay Kit-IBL 96-Well $606.00
27779A Human VEGFR-3/Flt-4 Assay Kit - IBL 96-Well $606.00
27171A Human VEGF Assay Kit - IBL 96-Well $606.00
18415A Anti-Human VEGF-C(103) Rabbit IgG Affinity Purify 10 ug $113.00
18415B Anti-Human VEGF-C(103) Rabbit IgG Affinity Purify 100 ug $499.00
18420A Anti-Rat VEGF (V-N) Rabbit IgG Affinity Purify 10 ug $113.00
18420B Anti-Rat VEGF (V-N) Rabbit IgG Affinity Purify 100 ug $499.00
 

 

 
 

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