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Products >  Cell_Biology_and_Epigenetics >  Cancer_and_Inflammation >  Tyrosine_Kinases_Focus

Detecting and Analyzing Tyrosine Kinase Proteins

Antibodies specific to c-Kit, PDGFR Beta, and more

Protein kinases catalyze the ATP-dependent addition of phosphate groups to substrate proteins, thereby regulating the activity, localization, and/or function of the protein. Members of the tyrosine kinase family specifically transfer phosphates to serine and threonine residues of target proteins. Tyrosine kinases can be categorized as either receptor tyrosine kinases, which function in transmembrane signaling, or non-receptor tyrosine kinases, which mediate intracellular signal transduction. Receptor tyrosine kinases are activated by ligand binding (e.g., growth factors, cytokines, and hormones), which triggers dimerization and trans-phosphorylation of a tyrosine residue in the intracellular domain. This phosphorylation creates high-affinity binding sites for Src homology 2 (SH2) and phosphotyrosine binding (PTB) domain-containing proteins. Phosphorylation of these proteins typically initiates a signal transduction cascade to mediate specific intracellular effects.

Tyrosine kinase-mediated signal transduction in response to internal and external stimuli plays a role in a large number of biological processes, including cellular growth, apoptosis, and cell migration (Figure 1). Mutation of or aberrant signaling through kinases can inappropriately, constitutively activate signaling pathways, thereby stimulating cellular proliferation and contributing to neoplastic growth. Blocking this activation with selective tyrosine kinase inhibitors is a being investigated as a possible cancer treatment strategy.

 

Receptor tyrosine kinase function
Figure 1. The functions of receptor tryosine kinases.

Tyrosine Kinase Antibodies

We offer monoclonal antibodies that recognize several tyrosine kinases, including c-Kit, PDGFR Beta, KDR, and Tie11. These antibodies can be used for Western blot, immunoprecipitation, and flow cytometry analysis. In addition, several of the antibodies can be used in neutralization assays.

1 Offered in partnership with IBL Co., Ltd., Japan. Products sold by Clontech Laboratories are for Research Use Only. Not for use in Diagnostic Procedures.

 

c-Kit

c-Kit is the receptor tyrosine kinase for stem cell factor (SCF). Activation of c-Kit by SCF triggers several signaling cascades, including the RAS/ERK, PI3-kinase, and JAK/STAT pathways. SCF/c-Kit is critical for the development and survival of hematopoietic stem cells. Deregulated c-Kit activity has been associated with several pathologies, including cancer and allergy.

Cat. #Product NameTargetAntigenApplications**
10349A, 10349B, 10349FAnti-Human c-Kit (12A8) Mouse IgG MoAbHuman c-Kit proteinExtracellular domain of human c-Kit recombinant proteinWB, IP, FC, NA
18101A, 18101BAnti-Human c-Kit (K963) Rabbit IgG PoAbHuman c-Kit proteinHuman c-Kit C-terminal synthetic peptideWB, IHC

 

 

PDFR Beta

Platelet-derived growth factor receptors (PDGFRs) are receptor tyrosine kinases that interact with members of the platelet-derived growth factor (PDGF) family. Binding of PDGF isoforms to PDGFR-beta initiates intracellular signaling through the MAPK, PI3K, and PKC-gamma pathways. A chromosomal translocation that that fuses the PDGFRB and ETV6 genes results in a chronic myeloproliferative disorder.

Cat. #Product NameTargetAntigenApplications**
10351A, 10351B, 10351FAnti-Human PDGFR beta (22B1) Mouse IgG MoAbHuman PDGFR-beta proteinExtracellular domain of human PDGFR-beta recombinant proteinWB, IP, FC, NA

 

 

KDR

Kinase insert domain receptor (KDR) (also known as Flk1) is a receptor tyrosine kinase that recognizes the vascular endothelial growth factor (VEGF) ligand and is the primary mediator of VEGF-induced proliferation, survival, migration, and differentiation in endothelial cells. Binding of VEGFA, VEGFC, or VEGFD to KDR stimulates several signaling cascades, including the protein kinase C (PKC), MAP kinase, and AKT1 kinase pathways.

Cat. #Product NameTargetAntigenApplications**
10347A, 10347B, 10347FAnti-Human KDR (23B31) Mouse IgG MoAbHuman KDR proteinExtracellular domain of human KDR recombinant proteinWB, IP, FC, NA
18435A, 18435BAnti-Human KDR (C) Rabbit IgG PoAbHuman KDR proteinHuman KDR C-terminal synthetic peptideWB

 

 

Tie1

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1) is a receptor tyrosine kinase that is expressed primarily in endothelial cells. Tie1 exhibits high homology to Tie2 and inhibits the binding of Tie-2 to the growth factor angiopoietin-1. Tie1 and Tie2 can be cleaved by various molecules including VEGF; proteolytic cleavage may be important for modulating angiopoietin-1 signaling. TIE-1 gene knockout mice form vasculature, but homozygotes develop pulmonary edema, suggesting a role for Tie-1 in maintenance of vascular structure.

Cat. #Product NameTargetAntigenApplications**
10353A, 10353B, 10353FAnti-Human Tie-1 (9C1) Mouse IgG MoAbHuman Tie1 proteinExtracellular domain of human Tie1 recombinant proteinWB, IP, FC
18435A, 18435BAnti-Human Tie-1 (N1125) Rabbit IgG PoAbHuman Tie1 proteinHuman Tie1 synthetic peptideWB, IHC

 

** WB: Western blot; IP: Immunoprecipitation; IHC: Immunohistochemistry; FC: Flow cytometry; NA: Neutralization Assay

 

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